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申请/专利权人：江苏省农业科学院

摘要：本发明公开了一种水稻ACCase突变型蛋白，所述ACCase突变型蛋白的氨基酸序列存在以下任意一种或几种突变：其对应于野生型水稻ACCase的氨基酸序列的第1796位、第1797位、第1835位、第1875位、第2010位、第2050位、第2060位、第2070位和第2106位发生突变。本发明还公开了一种核酸或基因，其编码所述的蛋白。本发明通过转基因方法将突变型基因在除草剂敏感水稻中表达，含有该突变蛋白的植物可以抗（耐）乙酰辅酶A羧化酶类除草剂。含有本发明的水稻ACCase蛋白的水稻三叶期幼苗在施用3mL喹禾灵L水（2倍推荐使用浓度）后，植株仍然正常生长发育和结实。

主权项：1.一种水稻ACCase突变型蛋白，所述ACCase突变型蛋白的氨基酸序列第2010位由色氨酸突变为甘氨酸或半胱氨酸，其氨基酸序列如SEQIDNO.10、SEQIDNO.12所示。

全文数据：具有除草剂抗性的ACCase突变型基因和蛋白及其应用技术领域本发明属于植物蛋白和植物抗除草剂领域，涉及具有除草剂抗性的ACCase突变型基因、蛋白及其应用；具体而言，本发明涉及水稻的乙酰辅酶A羧化酶ACCase突变基因及其编码的突变ACCase蛋白，该突变蛋白能赋予植物尤其水稻抗乙酰辅酶A羧化酶抑制剂类除草剂的特性。本发明公开了该突变蛋白的核苷酸序列和氨基酸序列，以及它们在植物抗除草剂领域中的应用。背景技术农田杂草是导致农作物减产的最主要的原因之一。与传统依靠栽培措施、人工除草和机械除草等方法相比，化学除草剂的使用是人们公认的农田除草技术中最可靠且最经济的手段。自2,4-D于20世纪40年代开始使用以来，除草剂工业已有60多年的发展历史，迄今已成功开发了一大批选择性除草剂。其中乙酰辅酶A羧化酶Acetyl-CoACarboxylase，简称ACCase是化学除草剂的一个重要靶标。ACCase酶存在于大多数的生物体中，主要存在于细菌、酵母、真菌、植物、动物和人类中。目前已经确定在植物体内有两种同工型的ACCase，主要位于质体和细胞溶胶中，它们的功能区域在结构上有很大的差别。大多数植物质体中的ACCase有4个亚基：一个是生物素羧基载体蛋白亚基biotincarboxylcarrier-protein，BCCP，一个是生物素羧化酶亚基biotincarboxylase，BC，以及羧基转移酶carboxyltransferase，CT的两个亚基CTα和CTβ，二者之间以共价键相连。大多数植物细胞溶胶中的ACCase与酵母、大鼠和人的ACCase属于同一类型，它的一条多肽链包含原核型ACCase所有的4个亚基，排列顺序是BC、BCCP、CTβ、CTα，形成了3个功能域CTβ和CTα算一个功能域。除草剂对ACCase的抑制主要是通过抑制羧基转移酶CT，造成酰基脂类生物合成受到抑制，影响细胞膜渗透性，造成代谢产物渗漏，致使植物死亡，从而达到防除禾本科杂草的目的。ACCase是一种以生物素为辅基的多功能酶。它利用ATP供给的能量催化由乙酰辅酶A生成丙二酰-CoA的羧基化反应，该反应是脂肪酸全合成的第一个关键步骤，也是限速步骤，这步由ACCase催化的反应实际上是由3个亚基组成的两步反应。第一步，首先由ATP提供能量给重碳酸，产生活化的羧基羧酸、磷酸复合物，然后在BC作用下将羧基连接到BCCP中生物素的脲环N1上，形成羧化的生物素羧基载体蛋白，该过程中由Mg2+或者Mn2+等的参与，碳酸氢盐为羧基供体。第二步，在CT作用下，将生物素脲环N1上的羧基转移至乙酰-CoA的甲基，产生丙二酰-CoA，该过程不需要其他的辅助能量。合成的丙二酰-CoA参与脂肪酸从头合成等生物过程。对于ACCase类抑制剂的研究始于20世纪70年代，ACCase类除草剂包括芳氧苯氧丙酸酯类Aryloxyphenoxypropanoates，APP、芳氧苯基环己二酮类Aryloxyphenylcy-clohexanedione，APCHD以及三酮类环己二酮Cyclictriketones,CTR。由于其选择性高，能够苗后防除一年或多年生禾本科杂草，且在植物体内传导，具有高效、低毒、施用期长、对后茬作物安全等优点，此类除草剂迅速发展、品种不断增多、使用面积迅速扩大，已成为世界第三类重要除草剂。但是随着长期和大面积的使用，杂草很快对这两种除草剂产生了抗性，目前，已在26个国家发现了高达35种杂草对这两类抑制剂产生了抗性。遗憾的是，ACCase抑制剂类除草剂的作用机理尚未确定，很难提前预测ACCase蛋白其它氨基酸位点的突变是否会产生除草剂抗性，只能依赖于科研人员长期、艰苦的实践探索，并凭借一些运气才可能发现ACCase蛋白新的除草剂抗性位点。发明内容技术问题：本发明所要解决的技术问题是提供一种可以赋予植物抗除草剂抗性的突变型蛋白。进一步地，本发明所要解决的技术问题是提供一种可以赋予植物抗耐乙酰辅酶A羧化酶类除草剂抗性的水稻ACCase突变型蛋白。进一步地，本发明所要解决的技术问题是提供一种可以赋予植物抗除草剂抗性的突变型基因。进一步地，本发明所要解决的技术问题是提供一种可以赋予植物抗耐乙酰辅酶A羧化酶类除草剂抗性的水稻ACCase突变型基因。为此，本发明提供了一种编码水稻ACCase突变型蛋白的核酸或基因。在一种实施方式中，本发明提供了一种水稻ACCase突变型蛋白，所述ACCase突变型蛋白的氨基酸序列存在以下任意一种或多种突变：其对应于水稻ACCase的氨基酸序列的第1796位、第1797位、第1835位、第1875位、第2010位、第2050位、第2060位、第2070位、第2106位发生突变。作为优选，本发明的水稻ACCase的氨基酸序列第1796位氨基酸由丙氨酸突变为甘氨酸、第1797位氨基酸由丙氨酸突变为脯氨酸、第1835位氨基酸由谷氨酰胺变为脯氨酸、第1875位氨基酸由缬氨酸变为苯丙氨酸、第2010位氨基酸由色氨酸变为甘氨酸、第2010位氨基酸由色氨酸变为半胱氨酸、第2050位氨基酸由谷氨酸变为甘氨酸、第2060位氨基酸由缬氨酸变为亮氨酸、第2070位氨基酸由丙氨酸变为缬氨酸、第2106位氨基酸由赖氨酸变为谷氨酸。优选地，本发明的水稻ACCase的氨基酸序列第1796位氨基酸为除了丙氨酸之外的其它氨基酸、第1797位氨基酸为除了丙氨酸之外的其它氨基酸、第1835位氨基酸为除了谷氨酰胺之外的其它氨基酸、第1875位氨基酸为除了缬氨酸之外的氨基酸、第2010位氨基酸为除了色氨酸之外的其它氨基酸、第2010位氨基酸为除了色氨酸之外的其它氨基酸、第2050位氨基酸为除了谷氨酸之外的其它氨基酸、第2060位氨基酸为除了缬氨酸之外的其它氨基酸、第2070位氨基酸为除了丙氨酸之外的其它氨基酸、第2106位氨基酸为除了赖氨酸之外的其它氨基酸。优选地，本发明的ACCase突变型蛋白发生突变的氨基酸包括但不仅限于以下几种突变，在以下位点突变成其他氨基酸的情况也在本发明的保护范围之内，例如：第1796位氨基酸由丙氨酸突变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、谷氨酸、天冬氨酸；第1797位氨基酸由丙氨酸突变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、谷氨酸、天冬氨酸；第1835位氨基酸由谷氨酰胺变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、丙氨酸、天冬氨酸；第1875位氨基酸由缬氨酸突变为丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、丙氨酸、谷氨酸、天冬氨酸；第2010位氨基酸由色氨酸变为苯丙氨酸、丝氨酸、酪氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、精氨酸、谷氨酰胺、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、丙氨酸、谷氨酸、天冬氨酸；第2050位氨基酸由谷氨酸变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、丙氨酸、天冬氨酸；第2060位氨基酸由缬氨酸变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、丙氨酸、谷氨酸、天冬氨酸；第2070位氨基酸由丙氨酸变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、谷氨酸、天冬氨酸；第2106位氨基酸由赖氨酸变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、丙氨酸、天冬氨酸。本发明还提供一种水稻ACCase突变型蛋白，包括：a其氨基酸序列选自由SEQIDNO.2、SEQIDNO.4、SEQIDNO.6、SEQIDNO.8、SEQIDNO.10、SEQIDNO.12、SEQIDNO.14、SEQIDNO.16、SEQIDNO.18和SEQIDNO.20组成的组中；或b在a中的氨基酸序列经过取代和或缺失和或添加一个或多个氨基酸，且具有乙酰辅酶A羧化酶活性的由a衍生的蛋白质。本发明还提供一种核酸或基因，其编码所述的蛋白。本发明所述的核酸或基因，包括：i编码所述的蛋白的核酸或基因；或ii在严格条件下与a限定的核苷酸序列杂交且编码具有乙酰辅酶A羧化酶活性的蛋白质的核苷酸序列；或iii其核苷酸序列选自由SEQIDNO:1、SEQIDNO:3、SEQIDNO:5、SEQIDNO:7、SEQIDNO:9、SEQIDNO:11、SEQIDNO:13、SEQIDNO:15、SEQIDNO:17和SEQIDNO:19组成的组中。所述严格条件为：在0.1×SSC、0.1％SDS的溶液中，65℃洗膜15分钟的高度严谨的杂交条件。本发明的ACCase突变型基因发生突变的核苷酸包括但不仅限于以下几种突变，在以下位点突变成其他核苷酸的情况也在本发明的保护范围之内，例如：A突变为T、C、G；或C突变为T、A、G；或T突变为A、C、G；或G突变为T、C、A等等均在本发明的保护范围之内。进一步地，本发明的水稻ACCase突变型基因，所述水稻ACCase基因存在以下一种或多种突变：在水稻ACCase基因的第5387位核苷酸、在水稻ACCase基因的第5389位核苷酸、在水稻ACCase基因的第5504位核苷酸、在水稻ACCase基因的第5623位核苷酸、在水稻ACCase基因的第6028位核苷酸、在水稻ACCase基因的第6030位核苷酸、在水稻ACCase基因的第6149位核苷酸、在水稻ACCase基因的第6178位核苷酸、在水稻ACCase基因的第6209位核苷酸、在水稻ACCase基因的第6316位核苷酸发生突变。作为优选，本发明的水稻ACCase突变型基因，所述水稻ACCase基因存在以下一种或多种突变：在水稻ACCase基因的第5387位核苷酸由C突变为G，在水稻ACCase基因的第5389位核苷酸由G突变为C，在水稻ACCase基因的第5504位核苷酸由A突变为C，在水稻ACCase基因的第5623位核苷酸由G突变为T，在水稻ACCase基因的第6028位核苷酸由T突变为G，在水稻ACCase基因的第6030位核苷酸由G突变为T，在水稻ACCase基因的第6149位核苷酸由A突变为C，在水稻ACCase基因的第6178位核苷酸由G突变为C，在水稻ACCase基因的第6209位核苷酸由C突变为T，在水稻ACCase基因的第6316位核苷酸由A突变为G。本发明还提供一种表达盒、重组载体或细胞，其含有所述的核酸或基因。本发明还提供所述的水稻ACCase突变型蛋白、核酸或基因，表达盒、重组载体或细胞在植物抗除草剂方面的应用。其中，上述除草剂是喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。本发明还提供获得具有除草剂抗性的植物的方法，包括如下步骤：1使植物包含所述核酸或基因；或2使植物表达所述水稻ACCase突变型蛋白。进一步地，所述方法包括crispr基因编辑、转基因、杂交、回交或无性繁殖步骤。本发明还提供一种鉴定植物的方法，其中所述植物是包含所述的核酸或基因的植物、表达所述的蛋白的植物或由所述的方法获得的植物，包括以下步骤：1测定所述植物是否包含所述的核酸或基因；或，2测定所述植物是否表达所述的蛋白。本发明还提供一种除草剂的防护剂，该防护剂包括所述的水稻ACCase突变型蛋白。本发明还提供一种控制杂草的方法，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含所述的核酸或基因或所述的表达盒、重组载体或细胞，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。本发明还提供一种用于保护植物免受由除草剂引起的损伤的方法，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含所述的核酸或基因或将所述的表达盒、重组载体导入植物，导入后的植物产生除草剂抗性蛋白，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。进一步地，本发明的野生型基因来源于水稻的嘉花1号。进一步地，本发明的嘉花1号的野生型植株不具有喹禾灵除草剂抗性，本发明的含有ACCase突变位点的转基因嘉花1号植株能赋予水稻植物增加对乙酰辅酶A羧化酶类除草剂的耐受性。其中，本发明的嘉花1号的突变型基因序列的编号及其对应的核苷酸和氨基酸序列分别是：嘉花1号的ACCase基因的第5387位核苷酸由C突变为G，1796位氨基酸由丙氨酸变为甘氨酸，其核苷酸序列如SEQIDNo:1所示，其氨基酸序列如SEQIDNo:2所示；其中，嘉花1号的ACCase基因的第5389位核苷酸由G变为C，1797位氨基酸由丙氨酸变为脯氨酸，其核苷酸序列如SEQIDNo:3所示，其氨基酸序列如SEQIDNo:4所示；其中，嘉花1号的ACCase基因的第5504位核苷酸由A变为C，1835位氨基酸由谷氨酰胺变为脯氨酸，其核苷酸序列如SEQIDNo:5所示，其氨基酸序列如SEQIDNo:6所示；其中，嘉花1号的ACCase基因的第5623位核苷酸由G变为T，1875位氨基酸由缬氨酸变为苯丙氨酸，其核苷酸序列如SEQIDNo:7所示，其氨基酸序列如SEQIDNo:8所示；其中，嘉花1号的ACCase基因的第6028位核苷酸由T变为G，2010位氨基酸由色氨酸变为甘氨酸，其核苷酸序列如SEQIDNo:9所示，其氨基酸序列如SEQIDNo:10所示；其中，嘉花1号的ACCase基因的第6030位核苷酸由G变为T，2010位氨基酸由色氨酸变为半胱氨酸，其核苷酸序列如SEQIDNo:11所示，其氨基酸序列如SEQIDNo:12所示；其中，嘉花1号的ACCase基因的第6149位核苷酸由A变为C，2050位氨基酸由谷氨酸变为甘氨酸，其核苷酸序列如SEQIDNo:13所示，其氨基酸序列如SEQIDNo:14所示；其中，嘉花1号的ACCase基因的第6178位核苷酸由G变为C，2060位氨基酸由缬氨酸变为亮氨酸，其核苷酸序列如SEQIDNo:15所示，其氨基酸序列如SEQIDNo:16所示；其中，嘉花1号的ACCase基因的第6209位核苷酸由C变为T，2070位氨基酸由丙氨酸变为缬氨酸，其核苷酸序列如SEQIDNo:17所示，其氨基酸序列如SEQIDNo:18所示；其中，嘉花1号的ACCase基因的第6316位核苷酸由A变为G，2106位氨基酸由赖氨酸Lys变为谷氨酸，其核苷酸序列如SEQIDNo:19所示，其氨基酸序列如SEQIDNo:20所示；其中，嘉花1号野生型ACCase基因核苷酸序列如SEQIDNo:21所示，其氨基酸序列如SEQIDNo:22所示。本发明还提供了获得具有除草剂抗性的植物的方法。进一步地，本发明提供了用于增强植物、植物组织、植物细胞对于至少一种除草剂的耐受性的方法，所述除草剂干扰ACCase酶的活性。因此，本发明中除草剂抗性指的是抗耐乙酰辅酶A羧化酶类除草剂。本发明所述的增强植物、植物组织或者植物细胞对除草剂的抗性方法可以通过转化或者杂交、自交和无性繁殖的方法实施，也可通过基因定点突变的方式获得，使改变的植株包括本发明的核苷酸序列SEQIDNo:1、SEQIDNo:3、SEQIDNo:5、SEQIDNo:7、SEQIDNo:9、SEQIDNo:11、SEQIDNo:13、SEQIDNo:15、SEQIDNo:17、SEQIDNo:19中的任意一种，所述的突变后的植株具有一种或者一种以上的上述突变后的核苷酸序列。本发明还提供了耐受除草剂的水稻植物，其表达含有抗除草剂突变位点的ACCase基因，该基因序列不同于野生型水稻植物的ACCase基因序列，是ACCase基因含有抗除草剂突变位点的序列。发明人通过定向突变的方法获得了上述携带有突变位点的水稻ACCase突变序列，并将其转入到野生型水稻品种，发现携带有ACCase突变位点的转基因水稻可以抗ACCase类除草剂，而野生型水稻对ACCase类除草剂表现敏感。本发明还提供了所述核酸或基因及蛋白在植物育种中的应用，用于培育具有除草剂抗性的植物，尤其是农作物，还提供了这些蛋白及其编码基因在转基因或者非转基因如水稻等作物中的应用。有益效果：相对于现有技术，本发明具备以下优点：1本发明首次通过同源比对的方式获得了具备除草剂抗性的水稻ACCase突变位点，并通过定点突变的方式扩增获得突变后的ACCase基因序列。2将ACCase突变序列转入到野生型水稻中，转基因水稻获得了ACCase酶类除草剂抗性。在田间喷施ACCase抑制剂类除草剂喹禾灵的实验结果表明，含有本发明的水稻ACCase突变蛋白的转基因水稻在施用2倍推荐使用浓度的乙酰辅酶A羧化酶抑制剂类除草剂后，植株仍然正常生长发育和结实，而野生型水稻幼苗在施用1倍推荐使用浓度的乙酰辅酶A羧化酶抑制剂类除草剂后，植株生长逐渐停止，叶片逐渐失绿、变浅黄色，植株不能拔节，最终整株死亡。附图说明图1示出了抗喹禾灵除草剂的含有ACCase突变位点的转基因水稻；图2示出了PCR扩增10个含有水稻ACCase突变位点的ACCase基因全长结果图；第1泳道为Marker；Marker分子量从上到下依次为15kb、8kb、5kb、3kb、2kb、1.5kb、1kb、750bp、500bp，第2泳道为野生型嘉花1号的DNA，第3泳道为含有抗除草剂突变位点ACCase-5387的DNA，第4泳道为含有抗除草剂突变位点ACCase-5389的DNA，第5泳道为含有抗除草剂突变位点ACCase-5504的DNA，第6泳道为含有抗除草剂突变位点ACCase-5623的DNA，第7泳道为含有抗除草剂突变位点ACCase-6028的DNA，第8泳道为含有抗除草剂突变位点ACCase-6030的DNA，第9泳道为含有抗除草剂突变位点ACCase-6149的DNA，第10泳道为含有抗除草剂突变位点ACCase-6178的DNA，第11泳道为含有抗除草剂突变位点ACCase-6209的DNA，第12泳道为含有抗除草剂突变位点ACCase-6316的DNA。具体实施方式下面将结合实施例对本发明的实施方案进行详细描述，但是本领域技术人员将会理解，下列实施例仅用于说明本发明，而不应视为限定本发明的范围。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市购获得的常规产品。实施例1：水稻ACCase基因突变位点的确定我们将大穗看麦娘Alopecurusmyosuroides黑草质体的ACCase序列GenbankAJ310767.1作为参考序列，与水稻基因组进行同源比对，发现与水稻基因组的ACCase基因XM_015783727.1的同源性最高。根据专利CN102946714B中提到的黑草ACCase可以抗除草剂的氨基酸突变位点，分别是1785丙氨酸突变为甘氨酸、1786丙氨酸突变为脯氨酸、1824谷氨酰胺变为脯氨酸、1864缬氨酸变为苯丙氨酸、1999色氨酸变为甘氨酸、1999色氨酸变为半胱氨酸、2039谷氨酸变为甘氨酸、2049缬氨酸变为亮氨酸、2059丙氨酸变为缬氨酸、2095赖氨酸变为谷氨酸作为参考，通过同源比对的方法，确定在水稻中ACCase基因突变的氨基酸位点分别为1796丙氨酸突变为甘氨酸、1797丙氨酸突变为脯氨酸、1835谷氨酰胺变为脯氨酸、1875缬氨酸变为苯丙氨酸、2010色氨酸变为甘氨酸、2010色氨酸变为半胱氨酸、2050谷氨酸变为甘氨酸、2060缬氨酸变为亮氨酸、2070丙氨酸变为缬氨酸、2106赖氨酸变为谷氨酸。根据氨基酸密码子对照表，确定对应核苷酸位点分别为5387C变为G、5389G变为C、5504A变为C、5623G变为T、6028T变为G、6030G变为T、6149A变为C、6178G变为C、6209C变为T、6316A变为G。实施例2：水稻嘉花1号野生型ACCase全长基因的获得选取嘉花1号野生型植株的叶片，提取基因组DNA，根据NCBI中日本晴ACCase基因NCBI：XM_015783727设计引物进行扩增，采用KODDNApolymerase聚合酶购自Toyobo公司扩增ACCase基因，其反应体系如下：补加无菌水至总体积50μlPCR扩增反应程序采用两步法，退火和延伸合为一起，采用68度。程序如下：预变性：94℃3min；35个循环：变性94℃10sec；延伸68℃10min；保温：72℃10min。取2μlPCR产物经1％琼脂糖凝胶电泳检测，发现有预期大小的片段后图2，剩余的PCR产物经PCR清洁试剂盒购自Axygen公司清洁回收后，克隆至pMD19-T载体购自Takara公司，然后转化大肠杆菌。每个转化随机挑取12个大肠杆菌单克隆进行PCR检测，取PCR结果呈阳性的6个单克隆，送南京一道生物科技有限公司测序，获得突变ACCase基因序列，该基因片段长度为13127bp，通过去除内含子后获得基因全长6984bp，嘉花1号野生型ACCase序列全长基因片段的测序结果如SEQIDNo:21所示。实施例3：水稻ACCase基因突变位点基因序列的获得利用点突变试剂盒扩增水稻ACCase基因的突变位点序列。首先，利用引物ACC-CF和ACC-CR，以嘉花1号cDNA为模板，克隆ACCase全长cDNA，连入TK007载体南京擎科生物科技有限公司，转化大肠杆菌TOP10菌株感受态细胞，挑选阳性转化子测序验证，获得正确单克隆，质粒命名为TK007-ACCase；第二，利用突变引物ACC-MutF和ACC-MutR，以TK007-ACCase为模版，进行PCR扩增，扩增产物限制性内切酶DpnI消化后，转入大肠杆菌DMT菌株感受态细胞，挑选阳性转化子测序验证，获得ACCase目标位点突变的质粒。利用突变引物ACC5387-MutF和ACC5387-MutR扩增ACCase-5387突变位点，其核苷酸序列的第5387位由C突变为G，导致其编码的氨基酸序列的第1796位由丙氨酸变为甘氨酸，其核苷酸序列如SEQIDNo:1所示，其编码的氨基酸序列如SEQIDNo:2所示；利用突变引物ACC5389-MutF和ACC5389-MutR扩增ACCase-5389突变位点，其核苷酸序列的第5389位由G突变为C，导致其编码的氨基酸序列的第1797位由丙氨酸变为脯氨酸，其核苷酸序列如SEQIDNo:3所示，其编码的氨基酸序列如SEQIDNo:4所示；利用突变引物ACC5504-MutF和ACC5504-MutR扩增ACCase-5504突变位点，其核苷酸序列的第5504位由A突变为C，导致其编码的氨基酸序列的第1835位由谷氨酰胺变为脯氨酸，其核苷酸序列如SEQIDNo:5所示，其编码的氨基酸序列如SEQIDNo:6所示；利用突变引物ACC5623-MutF和ACC5623-MutR扩增ACCase-5623突变位点，其核苷酸序列的第5623位由G突变为T，导致其编码的氨基酸序列的第1875位由缬氨酸变为苯丙氨酸，其核苷酸序列如SEQIDNo:7所示，其编码的氨基酸序列如SEQIDNo:8所示；利用突变引物ACC6028-MutF和ACC6028-MutR扩增ACCase-6028突变位点，其核苷酸序列第6028位由T突变为G，导致其编码的氨基酸序列的第2010位由色氨酸变为甘氨酸，其核苷酸序列如SEQIDNo:9所示，其编码的氨基酸序列如SEQIDNo:10所示。利用突变引物ACC6030-MutF和ACC6030-MutR扩增ACCase-6030突变位点，其核苷酸序列的第6030位由G突变为T，导致其编码的氨基酸序列的第2010位由色氨酸变为半胱氨酸，其核苷酸序列如SEQIDNo:11所示，其编码的氨基酸序列如SEQIDNo:12所示。利用突变引物ACC6149-MutF和ACC6149-MutR扩增ACCase-6149突变位点，其核苷酸序列的第6149位由A突变为C，导致其编码的氨基酸序列第2050位由谷氨酸变为甘氨酸，其核苷酸序列如SEQIDNo:13所示，其编码的氨基酸序列如SEQIDNo:14所示。利用突变引物ACC6178-MutF和ACC6178-MutR扩增ACCase-6178突变位点，其核苷酸序列第6178位由G突变为C，导致其编码的氨基酸序列第2060位由缬氨酸变为亮氨酸，其核苷酸序列如SEQIDNo:15所示，其编码的氨基酸序列如SEQIDNo:16所示。利用突变引物ACC6209-MutF和ACC6209-MutR扩增ACCase-6209突变位点，其核苷酸序列第6209位由C突变为T，导致其编码的氨基酸序列第2070位由丙氨酸变为缬氨酸，其核苷酸序列如SEQIDNo:17所示，其编码的氨基酸序列如SEQIDNo:18所示。利用突变引物ACC6316-MutF和ACC6316-MutR扩增ACCase-6316突变位点，其核苷酸序列第6316位由A突变为G，导致其编码的氨基酸序列第2106位由赖氨酸变为谷氨酸，其核苷酸序列如SEQIDNo:19所示，其编码的氨基酸序列如SEQIDNo:20所示。实施例4ACCase转基因水稻的获得及其抗除草剂验证通过Infuison反应，将突变的ACCase片段连入含有花椰菜花叶病毒35S启动子和35S终止子的植物表达载体中，分别转化野生型水稻。获得纯合并稳定表达含有ACCase突变位点的转基因水稻，并对转基因水稻检测除草剂的耐受性。将转基因水稻和野生型水稻分别播种，待水稻长至三叶期时，分别喷施乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵喷施浓度为施用1.5mL喹禾灵L水2倍推荐使用浓度、吡氟氯禾灵喷施浓度为施用1.5mL吡氟氯禾灵L水1倍推荐使用浓度、烯草酮喷施浓度为施用1.5mL烯草酮L水1倍推荐使用浓度、吡氟禾草灵喷施浓度为施用1.5mL吡氟禾草灵L水1倍推荐使用浓度，喷施一个月后调查枯死情况，发现野生型对照在喷施和以上同样浓度的乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵后，几乎停止生长，新长出来的叶片完全枯黄，并逐渐枯死。而含有ACCase突变位点的转基因水稻则全部对乙酰辅酶A羧化酶抑制剂类除草剂表现出抗的表型，其正常的生长发育几乎没有受到影响，最终正常拔节抽穗。表1中显示本发明转基因水稻对ACCase抑制性除草剂的抗性比野生型要高，伤害百分比要低60％以上。这表明我们获得的携带有ACCase突变位点的转基因水稻确实能够稳定遗传的获得抗乙酰辅酶A羧化酶抑制剂类除草剂的抗性。表1乙酰辅酶A羧化酶类除草剂对野生型和转基因水稻的伤害比率尽管本发明的具体实施方式已经得到详细描述，本领域技术人员将会理解，根据已经公开的所有教导，可以对那些细节进行各种修改和替换，这些均在本发明的保护范围内。本发明的全部范围由所附专利要求极其任何等同物给出。序列表110江苏省农业科学院120具有除草剂抗性的ACCase突变型基因和蛋白及其应用16044170SIPOSequenceListing1.021012116984212DNA213水稻Oryzasativa嘉花1号ACCase-5387突变核苷酸序列4001atgacatccacacatgtggcgacattgggagttggtgcccaggcacctcctcgtcaccag60aaaaagtcagctggcactgcatttgtatcatctgggtcatcaagaccctcataccgaaag120aatggtcagcgtactcggtcacttagggaagaaagcaatggaggagtgtctgattccaaa180aagcttaaccactctattcgccaaggtcttgctggcatcattgacctcccaaatgacgca240gcttcagaagttgatatttcacatggttccgaagatcccagggggcctacggtcccaggt300tcctaccaaatgaatgggattatcaatgaaacacataatgggaggcatgcttcagtctcc360aaggttgttgagttttgtacggcacttggtggcaaaacaccaattcacagtgtattagtg420gccaacaatggaatggcagcagctaagttcatgcggagtgtccgaacatgggctaatgat480acttttggatcagagaaggcaattcagctgatagctatggcaactccggaggatctgagg540ataaatgcagagcacatcagaattgccgatcaatttgtagaggtacctggtggaacaaac600aacaacaactatgcaaatgtccaactcatagtggagatagcagagagaacaggtgtttct660gctgtttggcctggttggggtcatgcatctgagaatcctgaacttccagatgcgctgact720gcaaaaggaattgtttttcttgggccaccagcatcatcaatgcatgcattaggagacaag780gttggctcagctctcattgctcaagcagctggagttccaacacttgcttggagtggatca840catgtggaagttcctctggagtgttgcttggactcaatacctgatgagatgtatagaaaa900gcttgtgttactaccacagaggaagcagttgcaagttgtcaggtggttggttatcctgcc960atgattaaggcatcttggggtggtggtggtaaaggaataaggaaggttcataatgatgat1020gaggttaggacattatttaagcaagttcaaggcgaagtacctggttccccaatatttatc1080atgaggctagctgctcagagtcgacatcttgaagttcagttgctttgtgatcaatatggc1140aacgtagcagcacttcacagtcgagattgcagtgtacaacggcgacaccaaaagataatc1200gaggaaggaccagttactgttgctcctcgtgagactgtgaaagagcttgagcaggcagca1260cggaggcttgctaaagctgtgggttatgttggtgctgctactgttgaatacctttacagc1320atggaaactggtgaatattattttctggaacttaatccacggctacaggttgagcatcct1380gtcactgagtggatagctgaagtaaatttgcctgcggctcaagttgctgttggaatgggt1440atacccctttggcagattccagagatcaggcgcttctacggaatgaaccatggaggaggc1500tatgacctttggaggaaaacagcagctctagcgactccatttaactttgatgaagtagat1560tctaaatggccaaaaggccactgcgtagctgttagaataactagcgaggatccagatgat1620gggtttaagcctactggtggaaaagtaaaggagataagtttcaagagtaaaccaaatgtt1680tgggcctatttctcagtaaagtctggtggaggcatccatgaattcgctgattctcagttc1740ggacatgtttttgcgtatggaactactagatcggcagcaataactaccatggctcttgca1800ctaaaagaggttcaaattcgtggagaaattcattcaaacgtagactacacagttgaccta1860ttaaatgcctcagattttagagaaaataagattcatactggttggctggataccaggata1920gccatgcgtgttcaagctgagaggcctccatggtatatttcagtcgttggaggggcttta1980tataaaacagtaactgccaacacggccactgtttctgattatgttggttatcttaccaag2040ggccagattccaccaaagcatatatcccttgtctatacgactgttgctttgaatatagat2100gggaaaaaatatacaatcgatactgtgaggagtggacatggtagctacagattgcgaatg2160aatggatcaacggttgacgcaaatgtacaaatattatgtgatggtgggcttttaatgcag2220ctggatggaaacagccatgtaatttatgctgaagaagaggccagtggtacacgacttctt2280attgatggaaagacatgcatgttacagaatgaccatgacccatcaaagttattagctgag2340acaccatgcaaacttcttcgtttcttggttgctgatggtgctcatgttgatgctgatgta2400ccatatgcggaagttgaggttatgaagatgtgcatgcccctcttatcacccgcttctggt2460gtcatacatgttgtaatgtctgagggccaagcaatgcaggctggtgatcttatagctagg2520ctggatcttgatgacccttctgctgttaagagagctgagccgttcgaagatacttttcca2580caaatgggtctccctattgctgcttctggccaagttcacaaattatgtgctgcaagtctg2640aatgcttgtcgaatgatccttgcggggtatgagcatgatattgacaaggttgtgccagag2700ttggtatactgcctagacactccggagcttcctttcctgcagtgggaggagcttatgtct2760gttttagcaactagacttccaagaaatcttaaaagtgagttggagggcaaatatgaggaa2820tacaaagtaaaatttgactctgggataatcaatgatttccctgccaatatgctacgagtg2880ataattgaggaaaatcttgcatgtggttctgagaaggagaaggctacaaatgagaggctt2940gttgagcctcttatgagcctactgaagtcatatgagggtgggagagaaagtcatgctcac3000tttgttgtcaagtccctttttgaggagtatctctatgttgaagaattgttcagtgatgga3060attcagtctgatgtgattgagcgtctgcgccttcaacatagtaaagacctacagaaggtc3120gtagacattgtgttgtcccaccagagtgttagaaataaaactaagctgatactaaaactc3180atggagagtctggtctatccaaatcctgctgcctacagggatcaattgattcgcttttct3240tcccttaatcacaaagcgtattacaagttggcacttaaagctagtgaacttcttgaacaa3300acaaaacttagtgagctccgtgcaagaatagcaaggagcctttcagagctggagatgttt3360actgaggaaagcaagggtctctccatgcataagcgagaaattgccattaaggagagcatg3420gaagatttagtcactgctccactgccagttgaagatgcgctcatttctttatttgattgt3480agtgatacaactgttcaacagagagtgattgagacttatatagctcgattataccagcct3540catcttgtaaaggacagtatcaaaatgaaatggatagaatcgggtgttattgctttatgg3600gaatttcctgaagggcattttgatgcaagaaatggaggagcggttcttggtgacaaaaga3660tggggtgccatggtcattgtcaagtctcttgaatcactttcaatggccattagatttgca3720ctaaaggagacatcacactacactagctctgagggcaatatgatgcatattgctttgttg3780ggtgctgataataagatgcatataattcaagaaagtggtgatgatgctgacagaatagcc3840aaacttcccttgatactaaaggataatgtaaccgatctgcatgcctctggtgtgaaaaca3900ataagtttcattgttcaaagagatgaagcacggatgacaatgcgtcgtaccttcctttgg3960tctgatgaaaagctttcttatgaggaagagccaattctccggcatgtggaacctcctctt4020tctgcacttcttgagttggacaagttgaaagtgaaaggatacaatgaaatgaagtatacc4080ccatcacgggatcgtcaatggcatatctacacacttagaaatactgaaaaccccaaaatg4140ttgcaccgggtatttttccgaacccttgtcaggcaacccagtgtatccaacaagttttct4200tcgggccagattggtgacatggaagttgggagtgctgaagaacctctgtcatttacatca4260accagcatattaagatctttgatgactgctatagaggaattggagcttcacgcaattaga4320actggccattcacacatgtatttgcatgtattgaaagaacaaaagcttcttgatcttgtt4380ccagtttcagggaatacagttttggatgttggtcaagatgaagctactgcatattcactt4440ttaaaagaaatggctatgaagatacatgaacttgttggtgcaagaatgcaccatctttct4500gtatgccaatgggaagtgaaacttaagttggactgcgatggtcctgccagtggtacctgg4560aggattgtaacaaccaatgttactagtcacacttgcactgtggatatctaccgtgagatg4620gaagataaagaatcacggaagttagtataccatcccgccactccggcggctggtcctctg4680catggtgtggcactgaataatccatatcagcctttgagtgtcattgatctcaaacgctgt4740tctgctaggaataatagaactacatactgctatgattttccactggcatttgaaactgca4800gtgaggaagtcatggtcctctagtacctctggtgcttctaaaggtgttgaaaatgcccaa4860tgttatgttaaagctacagagttggtatttgcggacaaacatgggtcatggggcactcct4920ttagttcaaatggaccggcctgctgggctcaatgacattggtatggtagcttggaccttg4980aagatgtccactcctgaatttcctagtggtagggagattattgttgttgcaaatgatatt5040acgttcagagctggatcatttggcccaagggaagatgcattttttgaagctgttaccaac5100ctagcctgtgagaagaaacttcctcttatttatttggcagcaaattctggtgctcgaatt5160ggcatagcagatgaagtgaaatcttgcttccgtgttgggtggtctgatgatggcagccct5220gaacgtgggtttcagtacatttatctaagcgaagaagactatgctcgtattggcacttct5280gtcatagcacataagatgcagctagacagtggtgaaattaggtgggttattgattctgtt5340gtgggcaaggaagatggacttggtgtggagaatatacatggaagtggtgctattgccagt5400gcttattctagggcatataaggagacatttacacttacatttgtgactggaagaactgtt5460ggaataggagcttatcttgctcgacttggcatccggtgcatacagcgtcttgaccagcct5520attattcttacaggctattctgcactgaacaagcttcttgggcgggaagtgtacagctcc5580cacatgcagttgggtggtcccaaaatcatggcaactaatggtgttgtccatcttactgtt5640tcagatgaccttgaaggcgtttctaatatattgaggtggctcagttatgttcctgcctac5700attggtggaccacttccagtaacaacaccgttggacccaccggacagacctgttgcatac5760attcctgagaactcgtgtgatcctcgagcggctatccgtggtgttgatgacagccaaggg5820aaatggttaggtggtatgtttgataaagacagctttgtggaaacatttgaaggttgggct5880aagacagtggttactggcagagcaaagcttggtggaattccagtgggtgtgatagctgtg5940gagactcagaccatgatgcaaactatccctgctgaccctggtcagcttgattcccgtgag6000caatctgttcctcgtgctggacaagtgtggtttccagattctgcaaccaagactgcgcag6060gcattgctggacttcaaccgtgaaggattacctctgttcatcctcgctaactggagaggc6120ttctctggtggacaaagagatctttttgaaggaattcttcaggctggctcgactattgtt6180gagaaccttaggacatacaatcagcctgcctttgtctacattcccatggctgcagagcta6240cgaggaggggcttgggttgtggttgatagcaagataaacccagaccgcattgagtgctat6300gctgagaggactgcaaaaggcaatgttctggaaccgcaagggttaattgagatcaagttc6360aggtcagaggaactccaggattgcatgagtcggcttgacccaacattaattgatctgaaa6420gcaaaactcgaagtagcaaataaaaatggaagtgctgacacaaaatcgcttcaagaaaat6480atagaagctcgaacaaaacagttgatgcctctatatactcagattgcgatacggtttgct6540gaattgcatgatacatccctcagaatggctgcgaaaggtgtgattaagaaagttgtggac6600tgggaagaatcacgatctttcttctataagagattacggaggaggatctctgaggatgtt6660cttgcaaaagaaattagagctgtagcaggtgagcagttttcccaccaaccagcaatcgag6720ctgatcaagaaatggtattcagcttcacatgcagctgaatgggatgatgacgatgctttt6780gttgcttggatggataaccctgaaaactacaaggattatattcaatatcttaaggctcaa6840agagtatcccaatccctctcaagtctttcagattccagctcagatttgcaagccctgcca6900cagggtctttccatgttactagataagatggatccctctagaagagctcaacttgttgaa6960gaaatcaggaaggtccttggttga698421022112327212PRT213水稻Oryzasativa嘉花1号ACCase-5387突变氨基酸序列4002MetThrSerThrHisValAlaThrLeuGlyValGlyAlaGlnAlaPro151015ProArgHisGlnLysLysSerAlaGlyThrAlaPheValSerSerGly202530SerSerArgProSerTyrArgLysAsnGlyGlnArgThrArgSerLeu354045ArgGluGluSerAsnGlyGlyValSerAspSerLysLysLeuAsnHis505560SerIleArgGlnGlyLeuAlaGlyIleIleAspLeuProAsnAspAla65707580AlaSerGluValAspIleSerHisGlySerGluAspProArgGlyPro859095ThrValProGlySerTyrGlnMetAsnGlyIleIleAsnGluThrHis100105110AsnGlyArgHisAlaSerValSerLysValValGluPheCysThrAla115120125LeuGlyGlyLysThrProIleHisSerValLeuValAlaAsnAsnGly130135140MetAlaAlaAlaLysPheMetArgSerValArgThrTrpAlaAsnAsp145150155160ThrPheGlySerGluLysAlaIleGlnLeuIleAlaMetAlaThrPro165170175GluAspLeuArgIleAsnAlaGluHisIleArgIleAlaAspGlnPhe180185190ValGluValProGlyGlyThrAsnAsnAsnAsnTyrAlaAsnValGln195200205LeuIleValGluIleAlaGluArgThrGlyValSerAlaValTrpPro210215220GlyTrpGlyHisAlaSerGluAsnProGluLeuProAspAlaLeuThr225230235240AlaLysGlyIleValPheLeuGlyProProAlaSerSerMetHisAla245250255LeuGlyAspLysValGlySerAlaLeuIleAlaGlnAlaAlaGlyVal260265270ProThrLeuAlaTrpSerGlySerHisValGluValProLeuGluCys275280285CysLeuAspSerIleProAspGluMetTyrArgLysAlaCysValThr290295300ThrThrGluGluAlaValAlaSerCysGlnValValGlyTyrProAla305310315320MetIleLysAlaSerTrpGlyGlyGlyGlyLysGlyIleArgLysVal325330335HisAsnAspAspGluValArgThrLeuPheLysGlnValGlnGlyGlu340345350ValProGlySerProIlePheIleMetArgLeuAlaAlaGlnSerArg355360365HisLeuGluValGlnLeuLeuCysAspGlnTyrGlyAsnValAlaAla370375380LeuHisSerArgAspCysSerValGlnArgArgHisGlnLysIleIle385390395400GluGluGlyProValThrValAlaProArgGluThrValLysGluLeu405410415GluGlnAlaAlaArgArgLeuAlaLysAlaValGlyTyrValGlyAla420425430AlaThrValGluTyrLeuTyrSerMetGluThrGlyGluTyrTyrPhe435440445LeuGluLeuAsnProArgLeuGlnValGluHisProValThrGluTrp450455460IleAlaGluValAsnLeuProAlaAlaGlnValAlaValGlyMetGly465470475480IleProLeuTrpGlnIleProGluIleArgArgPheTyrGlyMetAsn485490495HisGlyGlyGlyTyrAspLeuTrpArgLysThrAlaAlaLeuAlaThr500505510ProPheAsnPheAspGluValAspSerLysTrpProLysGlyHisCys515520525ValAlaValArgIleThrSerGluAspProAspAspGlyPheLysPro530535540ThrGlyGlyLysValLysGluIleSerPheLysSerLysProAsnVal545550555560TrpAlaTyrPheSerValLysSerGlyGlyGlyIleHisGluPheAla565570575AspSerGlnPheGlyHisValPheAlaTyrGlyThrThrArgSerAla580585590AlaIleThrThrMetAlaLeuAlaLeuLysGluValGlnIleArgGly595600605GluIleHisSerAsnValAspTyrThrValAspLeuLeuAsnAlaSer610615620AspPheArgGluAsnLysIleHisThrGlyTrpLeuAspThrArgIle625630635640AlaMetArgValGlnAlaGluArgProProTrpTyrIleSerValVal645650655GlyGlyAlaLeuTyrLysThrValThrAlaAsnThrAlaThrValSer660665670AspTyrValGlyTyrLeuThrLysGlyGlnIleProProLysHisIle675680685SerLeuValTyrThrThrValAlaLeuAsnIleAspGlyLysLysTyr690695700ThrIleAspThrValArgSerGlyHisGlySerTyrArgLeuArgMet705710715720AsnGlySerThrValAspAlaAsnValGlnIleLeuCysAspGlyGly725730735LeuLeuMetGlnLeuAspGlyAsnSerHisValIleTyrAlaGluGlu740745750GluAlaSerGlyThrArgLeuLeuIleAspGlyLysThrCysMetLeu755760765GlnAsnAspHisAspProSerLysLeuLeuAlaGluThrProCysLys770775780LeuLeuArgPheLeuValAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerGlyAlaIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleGlnArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyValValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValTrpPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGluGlyIleLeuGlnAlaGlySerThrIleValGluAsnLeuArg205020552060ThrTyrAsnGlnProAlaPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaLysGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly232521032116984212DNA213水稻Oryzasativa嘉花1号ACCase-5389突变核苷酸序列4003atgacatccacacatgtggcgacattgggagttggtgcccaggcacctcctcgtcaccag60aaaaagtcagctggcactgcatttgtatcatctgggtcatcaagaccctcataccgaaag120aatggtcagcgtactcggtcacttagggaagaaagcaatggaggagtgtctgattccaaa180aagcttaaccactctattcgccaaggtcttgctggcatcattgacctcccaaatgacgca240gcttcagaagttgatatttcacatggttccgaagatcccagggggcctacggtcccaggt300tcctaccaaatgaatgggattatcaatgaaacacataatgggaggcatgcttcagtctcc360aaggttgttgagttttgtacggcacttggtggcaaaacaccaattcacagtgtattagtg420gccaacaatggaatggcagcagctaagttcatgcggagtgtccgaacatgggctaatgat480acttttggatcagagaaggcaattcagctgatagctatggcaactccggaggatctgagg540ataaatgcagagcacatcagaattgccgatcaatttgtagaggtacctggtggaacaaac600aacaacaactatgcaaatgtccaactcatagtggagatagcagagagaacaggtgtttct660gctgtttggcctggttggggtcatgcatctgagaatcctgaacttccagatgcgctgact720gcaaaaggaattgtttttcttgggccaccagcatcatcaatgcatgcattaggagacaag780gttggctcagctctcattgctcaagcagctggagttccaacacttgcttggagtggatca840catgtggaagttcctctggagtgttgcttggactcaatacctgatgagatgtatagaaaa900gcttgtgttactaccacagaggaagcagttgcaagttgtcaggtggttggttatcctgcc960atgattaaggcatcttggggtggtggtggtaaaggaataaggaaggttcataatgatgat1020gaggttaggacattatttaagcaagttcaaggcgaagtacctggttccccaatatttatc1080atgaggctagctgctcagagtcgacatcttgaagttcagttgctttgtgatcaatatggc1140aacgtagcagcacttcacagtcgagattgcagtgtacaacggcgacaccaaaagataatc1200gaggaaggaccagttactgttgctcctcgtgagactgtgaaagagcttgagcaggcagca1260cggaggcttgctaaagctgtgggttatgttggtgctgctactgttgaatacctttacagc1320atggaaactggtgaatattattttctggaacttaatccacggctacaggttgagcatcct1380gtcactgagtggatagctgaagtaaatttgcctgcggctcaagttgctgttggaatgggt1440atacccctttggcagattccagagatcaggcgcttctacggaatgaaccatggaggaggc1500tatgacctttggaggaaaacagcagctctagcgactccatttaactttgatgaagtagat1560tctaaatggccaaaaggccactgcgtagctgttagaataactagcgaggatccagatgat1620gggtttaagcctactggtggaaaagtaaaggagataagtttcaagagtaaaccaaatgtt1680tgggcctatttctcagtaaagtctggtggaggcatccatgaattcgctgattctcagttc1740ggacatgtttttgcgtatggaactactagatcggcagcaataactaccatggctcttgca1800ctaaaagaggttcaaattcgtggagaaattcattcaaacgtagactacacagttgaccta1860ttaaatgcctcagattttagagaaaataagattcatactggttggctggataccaggata1920gccatgcgtgttcaagctgagaggcctccatggtatatttcagtcgttggaggggcttta1980tataaaacagtaactgccaacacggccactgtttctgattatgttggttatcttaccaag2040ggccagattccaccaaagcatatatcccttgtctatacgactgttgctttgaatatagat2100gggaaaaaatatacaatcgatactgtgaggagtggacatggtagctacagattgcgaatg2160aatggatcaacggttgacgcaaatgtacaaatattatgtgatggtgggcttttaatgcag2220ctggatggaaacagccatgtaatttatgct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tagaagagctcaacttgttgaa6960gaaatcaggaaggtccttggttga698421042112327212PRT213水稻Oryzasativa嘉花1号ACCase-5389突变氨基酸序列4004MetThrSerThrHisValAlaThrLeuGlyValGlyAlaGlnAlaPro151015ProArgHisGlnLysLysSerAlaGlyThrAlaPheValSerSerGly202530SerSerArgProSerTyrArgLysAsnGlyGlnArgThrArgSerLeu354045ArgGluGluSerAsnGlyGlyValSerAspSerLysLysLeuAsnHis505560SerIleArgGlnGlyLeuAlaGlyIleIleAspLeuProAsnAspAla65707580AlaSerGluValAspIleSerHisGlySerGluAspProArgGlyPro859095ThrValProGlySerTyrGlnMetAsnGlyIleIleAsnGluThrHis100105110AsnGlyArgHisAlaSerValSerLysValValGluPheCysThrAla115120125LeuGlyGlyLysThrProIleHisSerValLeuValAlaAsnAsnGly130135140MetAlaAlaAlaLysPheMetArgSerValArgThrTrpAlaAsnAsp145150155160ThrPheGlySerGluLysAlaIleGlnLeuIleAlaMetAlaThrPro165170175GluAspLeuArgIleAsnAlaGluHisIleArgIleAlaAspGlnPhe180185190ValGluValProGlyGlyThrAsnAsnAsnAsnTyrAlaAsnValGln195200205LeuIleValGluIleAlaGluArgThrGlyValSerAlaValTrpPro210215220GlyTrpGlyHisAlaSerGluAsnProGluLeuProAspAlaLeuThr225230235240AlaLysGlyIleValPheLeuGlyProProAlaSerSerMetHisAla245250255LeuGlyAspLysValGlySerAlaLeuIleAlaGlnAlaAlaGlyVal260265270ProThrLeuAlaTrpSerGlySerHisValGluValProLeuGluCys275280285CysLeuAspSerIleProAspGluMetTyrArgLysAlaCysValThr290295300ThrThrGluGluAlaValAlaSerCysGlnValValGlyTyrProAla305310315320MetIleLysAlaSerTrpGlyGlyGlyGlyLysGlyIleArgLysVal325330335HisAsnAspAspGluValArgThrLeuPheLysGlnValGlnGlyGlu340345350ValProGlySerProIlePheIleMetArgLeuAlaAlaGlnSerArg355360365HisLeuGluValGlnLeuLeuCysAspGlnTyrGlyAsnValAlaAla370375380LeuHisSerArgAspCysSerValGlnArgArgHisGlnLysIleIle385390395400GluGluGlyProValThrValAlaProArgGluThrValLysGluLeu405410415GluGlnAlaAlaArgArgLeuAlaLysAlaValGlyTyrValGlyAla420425430AlaThrValGluTyrLeuTyrSerMetGluThrGlyGluTyrTyrPhe435440445LeuGluLeuAsnProArgLeuGlnValGluHisProValThrGluTrp450455460IleAlaGluValAsnLeuProAlaAlaGlnValAlaValGlyMetGly465470475480IleProLeuTrpGlnIleProGluIleArgArgPheTyrGlyMetAsn485490495HisGlyGlyGlyTyrAspLeuTrpArgLysThrAlaAlaLeuAlaThr500505510ProPheAsnPheAspGluValAspSerLysTrpProLysGlyHisCys515520525ValAlaValArgIleThrSerGluAspProAspAspGlyPheLysPro530535540ThrGlyGlyLysValLysGluIleSerPheLysSerLysProAsnVal545550555560TrpAlaTyrPheSerValLysSerGlyGlyGlyIleHisGluPheAla565570575AspSerGlnPheGlyHisValPheAlaTyrGlyThrThrArgSerAla580585590AlaIleThrThrMetAlaLeuAlaLeuLysGluValGlnIleArgGly595600605GluIleHisSerAsnValAspTyrThrValAspLeuLeuAsnAlaSer610615620AspPheArgGluAsnLysIleHisThrGlyTrpLeuAspThrArgIle625630635640AlaMetArgValGlnAlaGluArgProProTrpTyrIleSerValVal645650655GlyGlyAlaLeuTyrLysThrValThrAlaAsnThrAlaThrValSer660665670AspTyrValGlyTyrLeuThrLysGlyGlnIleProProLysHisIle675680685SerLeuValTyrThrThrValAlaLeuAsnIleAspGlyLysLysTyr690695700ThrIleAspThrValArgSerGlyHisGlySerTyrArgLeuArgMet705710715720AsnGlySerThrValAspAlaAsnValGlnIleLeuCysAspGlyGly725730735LeuLeuMetGlnLeuAspGlyAsnSerHisValIleTyrAlaGluGlu740745750GluAlaSerGlyThrArgLeuLeuIleAspGlyLysThrCysMetLeu755760765GlnAsnAspHisAspProSerLysLeuLeuAlaGluThrProCysLys770775780LeuLeuArgPheLeuValAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerAlaProIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleGlnArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyValValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValTrpPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGluGlyIleLeuGlnAlaGlySerThrIleValGluAsnLeuArg205020552060ThrTyrAsnGlnProAlaPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaLysGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly232521052116984212DNA213水稻Oryzasativa嘉花1号ACCase-5504突变核苷酸序列4005atgacatccacacatgtggcgacattgggagttggtgcccaggcacctcctcgtcaccag60aaaaagtcagctggcactgcatttgtatcatctgggtcatcaagaccctcataccgaaag120aatggtcagcgtactcggtcacttagggaagaaagcaatggaggagtgtctgattccaaa180aagcttaaccactctattcgccaaggtcttgctggcatcattgacctcccaaatgacgca240gcttcagaagttgatatttcacatggttccgaagatcccagggggcctacggtcccaggt300tcctaccaaatgaatgggattatcaatgaaacacataatgggaggcatgcttcagtctcc360aaggttgttgagttttgtacggcacttggtggcaaaacaccaattcacagtgtattagtg420gccaacaatggaatggcagcagctaagttcatgcggagtgtccgaacatgggctaatgat480acttttggatcagagaaggcaattcagctgatagctatggcaactccggaggatctgagg540ataaatgcagagcacatcagaattgccgatcaatttgtagaggtacctggtggaacaaac600aacaacaactatgcaaatgtccaactcatagtggagatagcagagagaacaggtgtttct660gctgtttggcctggttggggtcatgcatctgagaatcctgaacttccagatgcgctgact720gcaaaaggaattgtttttcttgggccaccagcatcatcaatgcatgcattaggagacaag780gttggctcagctctcattgctcaagcagctggagttccaacacttgcttggagtggatca840catgtggaagttcctctggagtgttgcttggactcaatacctgatgagatgtatagaaaa900gcttgtgttactaccacagaggaagcagttgcaagttgtcaggtggttggttatcctgcc960atgattaaggcatcttggggtggtggtggtaaaggaataaggaaggttcataatgatgat1020gaggttaggacattatttaagcaagttcaaggcgaagtacctggttccccaatatttatc1080atgaggctagctgctcagagtcgacatcttgaagttcagttgctttgtgatcaatatggc1140aacgta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tatgtttgataaagacagctttgtggaaacatttgaaggttgggct5880aagacagtggttactggcagagcaaagcttggtggaattccagtgggtgtgatagctgtg5940gagactcagaccatgatgcaaactatccctgctgaccctggtcagcttgattcccgtgag6000caatctgttcctcgtgctggacaagtgtggtttccagattctgcaaccaagactgcgcag6060gcattgctggacttcaaccgtgaaggattacctctgttcatcctcgctaactggagaggc6120ttctctggtggacaaagagatctttttgaaggaattcttcaggctggctcgactattgtt6180gagaaccttaggacatacaatcagcctgcctttgtctacattcccatggctgcagagcta6240cgaggaggggcttgggttgtggttgatagcaagataaacccagaccgcattgagtgctat6300gctgagaggactgcaaaaggcaatgttctggaaccgcaagggttaattgagatcaagttc6360aggtcagaggaactccaggattgcatgagtcggcttgacccaacattaattgatctgaaa6420gcaaaactcgaagtagcaaataaaaatggaagtgctgacacaaaatcgcttcaagaaaat6480atagaagctcgaacaaaacagttgatgcctctatatactcagattgcgatacggtttgct6540gaattgcatgatacatccctcagaatggctgcgaaaggtgtgattaagaaagttgtggac6600tgggaagaatcacgatctttcttctataagagattacggaggaggatctctgaggatgtt6660cttgcaaaagaaattagagctgtagcaggtgagcagttttcccaccaaccagcaatcgag6720ctgatcaagaaatggtattcagcttcacatgcagctgaatgggatgatgacgatgctttt6780gttgcttggatggataaccctgaaaactacaaggattatattcaatatcttaaggctcaa6840agagtatcccaatccctctcaagtctttcagattccagctcagatttgcaagccctgcca6900cagggtctttccatgttactagataagatggatccctctagaagagctcaacttgttgaa6960gaaatcaggaaggtccttggttga698421062112327212PRT213水稻Oryzasativa嘉花1号ACCase-5504突变氨基酸序列4006MetThrSerThrHisValAlaThrLeuGlyValGlyAlaGlnAlaPro151015ProArgHisGlnLysLysSerAlaGlyThrAlaPheValSerSerGly202530SerSerArgProSerTyrArgLysAsnGlyGlnArgThrArgSerLeu354045ArgGluGluSerAsnGlyGlyValSerAspSerLysLysLeuAsnHis505560SerIleArgGlnGlyLeuAlaGlyIleIleAspLeuProAsnAspAla65707580AlaSerGluValAspIleSerHisGlySerGluAspProArgGlyPro859095ThrValProGlySerTyrGlnMetAsnGlyIleIleAsnGluThrHis100105110AsnGlyArgHisAlaSerValSerLysValValGluPheCysThrAla115120125LeuGlyGlyLysThrProIleHisSerValLeuValAlaAsnAsnGly130135140MetAlaAlaAlaLysPheMetArgSerValArgThrTrpAlaAsnAsp145150155160ThrPheGlySerGluLysAlaIleGlnLeuIleAlaMetAlaThrPro165170175GluAspLeuArgIleAsnAlaGluHisIleArgIleAlaAspGlnPhe180185190ValGluValProGlyGlyThrAsnAsnAsnAsnTyrAlaAsnValGln195200205LeuIleValGluIleAlaGluArgThrGlyValSerAlaValTrpPro210215220GlyTrpGlyHisAlaSerGluAsnProGluLeuProAspAlaLeuThr225230235240AlaLysGlyIleValPheLeuGlyProProAlaSerSerMetHisAla245250255LeuGlyAspLysValGlySerAlaLeuIleAlaGlnAlaAlaGlyVal260265270ProThrLeuAlaTrpSerGlySerHisValGluValProLeuGluCys275280285CysLeuAspSerIleProAspGluMetTyrArgLysAlaCysValThr290295300ThrThrGluGluAlaValAlaSerCysGlnValValGlyTyrProAla305310315320MetIleLysAlaSerTrpGlyGlyGlyGlyLysGlyIleArgLysVal325330335HisAsnAspAspGluValArgThrLeuPheLysGlnValGlnGlyGlu340345350ValProGlySerProIlePheIleMetArgLeuAlaAlaGlnSerArg355360365HisLeuGluValGlnLeuLeuCysAspGlnTyrGlyAsnValAlaAla370375380LeuHisSerArgAspCysSerValGlnArgArgHisGlnLysIleIle385390395400GluGluGlyProValThrValAlaProArgGluThrValLysGluLeu405410415GluGlnAlaAlaArgArgLeuAlaLysAlaValGlyTyrValGlyAla420425430AlaThrValGluTyrLeuTyrSerMetGluThrGlyGluTyrTyrPhe435440445LeuGluLeuAsnProArgLeuGlnValGluHisProValThrGluTrp450455460IleAlaGluValAsnLeuProAlaAlaGlnValAlaValGlyMetGly465470475480IleProLeuTrpGlnIleProGluIleArgArgPheTyrGlyMetAsn485490495HisGlyGlyGlyTyrAspLeuTrpArgLysThrAlaAlaLeuAlaThr500505510ProPheAsnPheAspGluValAspSerLysTrpProLysGlyHisCys515520525ValAlaValArgIleThrSerGluAspProAspAspGlyPheLysPro530535540ThrGlyGlyLysValLysGluIleSerPheLysSerLysProAsnVal545550555560TrpAlaTyrPheSerValLysSerGlyGlyGlyIleHisGluPheAla565570575AspSerGlnPheGlyHisValPheAlaTyrGlyThrThrArgSerAla580585590AlaIleThrThrMetAlaLeuAlaLeuLysGluValGlnIleArgGly595600605GluIleHisSerAsnValAspTyrThrValAspLeuLeuAsnAlaSer610615620AspPheArgGluAsnLysIleHisThrGlyTrpLeuAspThrArgIle625630635640AlaMetArgValGlnAlaGluArgProProTrpTyrIleSerValVal645650655GlyGlyAlaLeuTyrLysThrValThrAlaAsnThrAlaThrValSer660665670AspTyrValGlyTyrLeuThrLysGlyGlnIleProProLysHisIle675680685SerLeuValTyrThrThrValAlaLeuAsnIleAspGlyLysLysTyr690695700ThrIleAspThrValArgSerGlyHisGlySerTyrArgLeuArgMet705710715720AsnGlySerThrValAspAlaAsnValGlnIleLeuCysAspGlyGly725730735LeuLeuMetGlnLeuAspGlyAsnSerHisValIleTyrAlaGluGlu740745750GluAlaSerGlyThrArgLeuLeuIleAspGlyLysThrCysMetLeu755760765GlnAsnAspHisAspProSerLysLeuLeuAlaGluThrProCysLys770775780LeuLeuArgPheLeuValAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerAlaAlaIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleAspArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyValValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValTrpPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGluGlyIleLeuGlnAlaGlySerThrIleValGluAsnLeuArg205020552060ThrTyrAsnGlnProAlaPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaLysGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly232521072116984212DNA213水稻Oryzasativa嘉花1号ACCase-5623突变核苷酸序列4007atgacatccacacatgtggcgacattggg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cgctgt4740tctgctaggaataatagaactacatactgctatgattttccactggcatttgaaactgca4800gtgaggaagtcatggtcctctagtacctctggtgcttctaaaggtgttgaaaatgcccaa4860tgttatgttaaagctacagagttggtatttgcggacaaacatgggtcatggggcactcct4920ttagttcaaatggaccggcctgctgggctcaatgacattggtatggtagcttggaccttg4980aagatgtccactcctgaatttcctagtggtagggagattattgttgttgcaaatgatatt5040acgttcagagctggatcatttggcccaagggaagatgcattttttgaagctgttaccaac5100ctagcctgtgagaagaaacttcctcttatttatttggcagcaaattctggtgctcgaatt5160ggcatagcagatgaagtgaaatcttgcttccgtgttgggtggtctgatgatggcagccct5220gaacgtgggtttcagtacatttatctaagcgaagaagactatgctcgtattggcacttct5280gtcatagcacataagatgcagctagacagtggtgaaattaggtgggttattgattctgtt5340gtgggcaaggaagatggacttggtgtggagaatatacatggaagtgctgctattgccagt5400gcttattctagggcatataaggagacatttacacttacatttgtgactggaagaactgtt5460ggaataggagcttatcttgctcgacttggcatccggtgcatacagcgtcttgaccagcct5520attattcttacaggctattctgcactgaacaagcttcttgggcgggaagtgtacagctcc5580cacatgcagttgggtggtcccaaaatcatggcaactaatggttttgtccatcttactgtt5640tcagatgaccttgaaggcgtttctaatatattgaggtggctcagttatgttcctgcctac5700attggtggaccacttccagtaacaacaccgttggacccaccggacagacctgttgcatac5760attcctgagaactcgtgtgatcctcgagcggctatccgtggtgttgatgacagccaaggg5820aaatggttaggtggtatgtttgataaagacagctttgtggaaacatttgaaggttgggct5880aagacagtggttactggcagagcaaagcttggtggaattccagtgggtgtgatagctgtg5940gagactcagaccatgatgcaaactatccctgctgaccctggtcagcttgattcccgtgag6000caatctgttcctcgtgctggacaagtgtggtttccagattctgcaaccaagactgcgcag6060gcattgctggacttcaaccgtgaaggattacctctgttcatcctcgctaactggagaggc6120ttctctggtggacaaagagatctttttgaaggaattcttcaggctggctcgactattgtt6180gagaaccttaggacatacaatcagcctgcctttgtctacattcccatggctgcagagcta6240cgaggaggggcttgggttgtggttgatagcaagataaacccagaccgcattgagtgctat6300gctgagaggactgcaaaaggcaatgttctggaaccgcaagggttaattgagatcaagttc6360aggtcagaggaactccaggattgcatgagtcggcttgacccaacattaattgatctgaaa6420gcaaaactcgaagtagcaaataaaaatggaagtgctgacacaaaatcgcttcaagaaaat6480atagaagctcgaacaaaacagttgatgcctctatatactcagattgcgatacggtttgct6540gaattgcatgatacatccctcagaatggctgcgaaaggtgtgattaagaaagttgtggac6600tgggaagaatcacgatctttcttctataagagattacggaggaggatctctgaggatgtt6660cttgcaaaagaaattagagctgtagcaggtgagcagttttcccaccaaccagcaatcgag6720ctgatcaagaaatggtattcagcttcacatgcagctgaatgggatgatgacgatgctttt6780gttgcttggatggataaccctgaaaactacaaggattatattcaatatcttaaggctcaa6840agagtatcccaatccctctcaagtctttcagattccagctcagatttgcaagccctgcca6900cagggtctttccatgttactagataagatggatccctctagaagagctcaacttgttgaa6960gaaatcaggaaggtccttggttga698421082112327212PRT213水稻Oryzasativa嘉花1号ACCase-5623突变氨基酸序列4008MetThrSerThrHisValAlaThrLeuGlyValGlyAlaGlnAlaPro151015ProArgHisGlnLysLysSerAlaGlyThrAlaPheValSerSerGly202530SerSerArgProSerTyrArgLysAsnGlyGlnArgThrArgSerLeu354045ArgGluGluSerAsnGlyGlyValSerAspSerLysLysLeuAsnHis505560SerIleArgGlnGlyLeuAlaGlyIleIleAspLeuProAsnAspAla65707580AlaSerGluValAspIleSerHisGlySerGluAspProArgGlyPro859095ThrValProGlySerTyrGlnMetAsnGlyIleIleAsnGluThrHis100105110AsnGlyArgHisAlaSerValSerLysValValGluPheCysThrAla115120125LeuGlyGlyLysThrProIleHisSerValLeuValAlaAsnAsnGly130135140MetAlaAlaAlaLysPheMetArgSerValArgThrTrpAlaAsnAsp145150155160ThrPheGlySerGluLysAlaIleGlnLeuIleAlaMetAlaThrPro165170175GluAspLeuArgIleAsnAlaGluHisIleArgIleAlaAspGlnPhe180185190ValGluValProGlyGlyThrAsnAsnAsnAsnTyrAlaAsnValGln195200205LeuIleValGluIleAlaGluArgThrGlyValSerAlaValTrpPro210215220GlyTrpGlyHisAlaSerGluAsnProGluLeuProAspAlaLeuThr225230235240AlaLysGlyIleValPheLeuGlyProProAlaSerSerMetHisAla245250255LeuGlyAspLysValGlySerAlaLeuIleAlaGlnAlaAlaGlyVal260265270ProThrLeuAlaTrpSerGlySerHisValGluValProLeuGluCys275280285CysLeuAspSerIleProAspGluMetTyrArgLysAlaCysValThr290295300ThrThrGluGluAlaValAlaSerCysGlnValValGlyTyrProAla305310315320MetIleLysAlaSerTrpGlyGlyGlyGlyLysGlyIleArgLysVal325330335HisAsnAspAspGluValArgThrLeuPheLysGlnValGlnGlyGlu340345350ValProGlySerProIlePheIleMetArgLeuAlaAlaGlnSerArg355360365HisLeuGluValGlnLeuLeuCysAspGlnTyrGlyAsnValAlaAla370375380LeuHisSerArgAspCysSerValGlnArgArgHisGlnLysIleIle385390395400GluGluGlyProValThrValAlaProArgGluThrValLysGluLeu405410415GluGlnAlaAlaArgArgLeuAlaLysAlaValGlyTyrValGlyAla420425430AlaThrValGluTyrLeuTyrSerMetGluThrGlyGluTyrTyrPhe435440445LeuGluLeuAsnProArgLeuGlnValGluHisProValThrGluTrp450455460IleAlaGluValAsnLeuProAlaAlaGlnValAlaValGlyMetGly465470475480IleProLeuTrpGlnIleProGluIleArgArgPheTyrGlyMetAsn485490495HisGlyGlyGlyTyrAspLeuTrpArgLysThrAlaAlaLeuAlaThr500505510ProPheAsnPheAspGluValAspSerLysTrpProLysGlyHisCys515520525ValAlaValArgIleThrSerGluAspProAspAspGlyPheLysPro530535540ThrGlyGlyLysValLysGluIleSerPheLysSerLysProAsnVal545550555560TrpAlaTyrPheSerValLysSerGlyGlyGlyIleHisGluPheAla565570575AspSerGlnPheGlyHisValPheAlaTyrGlyThrThrArgSerAla580585590AlaIleThrThrMetAlaLeuAlaLeuLysGluValGlnIleArgGly595600605GluIleHisSerAsnValAspTyrThrValAspLeuLeuAsnAlaSer610615620AspPheArgGluAsnLysIleHisThrGlyTrpLeuAspThrArgIle625630635640AlaMetArgValGlnAlaGluArgProProTrpTyrIleSerValVal645650655GlyGlyAlaLeuTyrLysThrValThrAlaAsnThrAlaThrValSer660665670AspTyrValGlyTyrLeuThrLysGlyGlnIleProProLysHisIle675680685SerLeuValTyrThrThrValAlaLeuAsnIleAspGlyLysLysTyr690695700ThrIleAspThrValArgSerGlyHisGlySerTyrArgLeuArgMet705710715720AsnGlySerThrValAspAlaAsnValGlnIleLeuCysAspGlyGly725730735LeuLeuMetGlnLeuAspGlyAsnSerHisValIleTyrAlaGluGlu740745750GluAlaSerGlyThrArgLeuLeuIleAspGlyLysThrCysMetLeu755760765GlnAsnAspHisAspProSerLysLeuLeuAlaGluThrProCysLys770775780LeuLeuArgPheLeuValAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerAlaAlaIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleGlnArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyPheValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValTrpPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGluGlyIleLeuGlnAlaGlySerThrIleValGluAsnLeuArg205020552060ThrTyrAsnGlnProAlaPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaLysGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly232521092116984212DNA213水稻Oryzasativa嘉花1号ACCase-6028突变核苷酸序列4009atgacatccacacatgtggcgacattgggagttggtgcccaggcacctcctcgtcaccag60aaaaagtcagctggcactgcatttgtatcatctgggtcatcaagaccctcataccgaaag120aatggtcagcgtactcggtcacttagggaagaaagcaatggaggagtgtctgattccaaa180aagcttaaccactctattcgccaaggtcttgctggcatcattgacctcccaaatgacgca240gcttcagaagttgatatttcacatggttccgaagatcccagggggcctacggtcccaggt300tcctaccaaatgaatgggattatcaatgaaacacataatgggaggcatgcttcagtctcc360aaggttgttgagttttgtacggcacttggtggcaaaacaccaattcacagtgtattagtg420gccaacaatggaatggcagcagctaagttcatgcggagtgtccgaacatgggctaatgat480acttttggatcagagaaggcaattcagctgatagctatggcaactccggaggatctgagg540ataaatgcagagcacatcagaattgccgatcaatttgtagaggtacctggtggaacaaac600aacaacaactatgcaaatgtccaactcatagtggagatagcagagagaacaggtgtttct660gctgtttggcctggttggggtcatgcatctgagaatcctgaacttccagatgcgctgact720gcaaaaggaattgtttttcttgggccaccagcatcatcaatgcatgcattaggagacaag780gttggctcagctctcattgctcaagcagctggagttccaacacttgcttggagtggatca840catgtggaagttcctctggagtgttgcttggactcaatacctgatgagatgtatagaaaa900gcttgtgttactaccacagaggaagcagttgcaagttgtcaggtggttggttatcctgcc960atgattaaggcatcttggggtggtggtggtaaaggaataaggaaggttcataatgatgat1020gaggttaggacattatttaagcaagttcaaggcgaagtacctggttccccaatatttatc1080atgaggctagctgctcagagtcgacatcttgaagttcagttgctttgtgatcaatatggc1140aacgtagcagcacttcacagtcgagattgcagtgtacaacggcgacaccaaaagataatc1200gaggaaggaccagttactgttgctcctcgtgagactgtgaaagagcttgagcaggcagca1260cggaggcttgctaaagctgtgggttatgttggtgctgctactgttgaatacctttacagc1320atggaaactggtgaatattattttctggaacttaatccacggctacaggttgagcatcct1380gtcactgagtggatagctgaagtaaatttgcctgcggctcaagttgctgttggaatgggt1440atacccctttggcagattccagagatcaggcgcttctacggaatgaaccatggaggaggc1500tatgacctttggaggaaaacagcagctctagcgactccatttaactttgatgaagtagat1560tctaaatggccaaaaggccactgcgtagctgttagaataactagcgaggatccagatgat1620gggtttaagcctactggtggaaaagtaaaggagataagtttcaagagtaaaccaaatgtt1680tgggcctatttctcagtaaagtctggtggaggcatccatgaattcgctgattctcagttc1740ggacatgtttttgcgtatggaactactagatcggcagcaataactaccatggctcttgca1800ctaaaagaggttcaaattcgtggagaaattcattcaaacgtagactacacagttgaccta1860ttaaatgcctcagattttagagaaaataagattcatactggttggctggataccaggata1920gccatgcgtgttcaagctgagaggcctccatggtatatttcagtcgttggaggggcttta1980tataaaacagtaactgccaacacggccactgtttctgattatgttggttatcttaccaag2040ggccagattccaccaaagcatatatcccttgtctatacgactgttgctttgaatatagat2100gggaaaaaatatacaatcgatactgtgaggagtggacatggtagctacagattgcgaatg2160aatggatcaacggttgacgcaaatgtacaaatattatgtgatggtgggcttttaatgcag2220ctggatggaaacagccatgtaatttatgctgaagaagaggccagtggtacacgacttctt2280attgatggaaagacatgcatgttacagaatgaccatgacccatcaaagttattagctgag2340acaccatgcaaacttcttcgtttcttggttgctgatggtgctcatgttgatgctgatgta2400ccatatgcggaagttgaggttatgaagatgtgcatgcccctcttatcacccgcttctggt2460gtcatacatgttgtaatgtctgagggccaagcaatgcaggctggtgatcttatagctagg2520ctggatcttgatgacccttctgctgttaagagagctgagccgttcgaagatacttttcca2580caaatgggtctccctattgctgcttctggccaagttcacaaattatgtgctgcaagtctg2640aatgcttgtcgaatgatccttgcggggtatgagcatgatattgacaaggttgtgccagag2700ttggtatactgcctagacactccggagcttcctttcctgcagtgggaggagcttatgtct2760gttttagcaactagacttccaagaaatcttaaaagtgagttggagggcaaatatgaggaa2820tacaaagtaaaatttgactctgggataatcaatgatttccctgccaatatgctacgagtg2880ataattgaggaaaatcttgcatgtggttctgagaaggagaaggctacaaatgagaggctt2940gttgagcctcttatgagcctactgaagtcatatgagggtgggagagaaagtcatgctcac3000tttgttgtcaagtccctttttgaggagtatctctatgttgaagaattgttcagtgatgga3060attcagtctgatgtgattgagcgtctgcgccttcaacatagtaaagacctacagaaggtc3120gtagacattgtgttgtcccaccagagtgttagaaataaaactaagctgatactaaaactc3180atggagagtctggtctatccaaatcctgctgcctacagggatcaattgattcgcttttct3240tcccttaatcacaaagcgtattacaagttggcacttaaagctagtgaacttcttgaacaa3300acaaaacttagtgagctccgtgcaagaatagcaaggagcctttcagagctggagatgttt3360actgaggaaagcaagggtctctccatgcataagcgagaaattgccattaaggagagcatg3420gaagatttagtcactgctccactgccagttgaagatgcgctcatttctttatttgattgt3480agtgatacaactgttcaacagagagtgattgagacttatatagctcgattataccagcct3540catcttgtaaaggacagtatcaaaatgaaatggatagaatcgggtgttattgctttatgg3600gaatttcctgaagggcattttgatgcaagaaatggaggagcggttcttggtgacaaaaga3660tggggtgccatggtcattgtcaagtctcttgaatcactttcaatggccattagatttgca3720ctaaaggagacatcacactacactagctctgagggcaatatgatgcatattgctttgttg3780ggtgctgataataagatgcatataattcaagaaagtggtgatgatgctgacagaatagcc3840aaacttcccttgatactaaaggataatgtaaccgatctgcatgcctctggtgtgaaaaca3900ataagtttcattgttcaaagagatgaagcacggatgacaatgcgtcgtaccttcctttgg3960tctgatgaaaagctttcttatgaggaagagccaattctccggcatgtggaacctcctctt4020tctgcacttcttgagttggacaagttgaaagtgaaaggatacaatgaaatgaagtatacc4080ccatcacgggatcgtcaatggcatatctacacacttagaaatactgaaaaccccaaaatg4140ttgcaccgggtatttttccgaacccttgtcaggcaacccagtgtatccaacaagttttct4200tcgggccagattggtgacatggaagttgggagtgctgaagaacctctgtcatttacatca4260accagcatattaagatctttgatgactgctatagaggaattggagcttcacgcaattaga4320actggccattcacacatgtatttgcatgtattgaaagaacaaaagcttcttgatcttgtt4380ccagtttcagggaatacagttttggatgttggtcaagatgaagctactgcatattcactt4440ttaaaagaaatggctatgaagatacatgaacttgttggtgcaagaatgcaccatctttct4500gtatgccaatgggaagtgaaacttaagttggactgcgatggtcctgccagtggtacctgg4560aggattgtaacaaccaatgttactagtcacacttgcactgtggatatctaccgtgagatg4620gaagataaagaatcacggaagttagtataccatcccgccactccggcggctggtcctctg4680catggtgtggcactgaataatccatatcagcctttgagtgtcattgatctcaaacgctgt4740tctgctaggaataatagaactacatactgctatgattttccactggcatttgaaactgca4800gtgaggaagtcatggtcctctagtacctctggtgcttctaaaggtgttgaaaatgcccaa4860tgttatgttaaagctacagagttggtatttgcggacaaacatgggtcatggggcactcct4920ttagttcaaatggaccggcctgctgggctcaatgacattggtatggtagcttggaccttg4980aagatgtccactcctgaatttcctagtggtagggagattattgttgttgcaaatgatatt5040acgttcagagctggatcatttggcccaagggaagatgcattttttgaagctgttaccaac5100ctagcctgtgagaagaaacttcctcttatttatttggcagcaaattctggtgctcgaatt5160ggcatagcagatgaagtgaaatcttgcttccgtgttgggtggtctgatgatggcagccct5220gaacgtgggtttcagtacatttatctaagcgaagaagactatgctcgtattggcacttct5280gtcatagcacataagatgcagctagacagtggtgaaattaggtgggttattgattctgtt5340gtgggcaaggaagatggacttggtgtggagaatatacatggaagtgctgctattgccagt5400gcttattctagggcatataaggagacatttacacttacatttgtgactggaagaactgtt5460ggaataggagcttatcttgctcgacttggcatccggtgcatacagcgtcttgaccagcct5520attattcttacaggctattctgcactgaacaagcttcttgggcgggaagtgtacagctcc5580cacatgcagttgggtggtcccaaaatcatggcaactaatggtgttgtccatcttactgtt5640tcagatgaccttgaaggcgtttctaatatattgaggtggctcagttatgttcctgcctac5700attggtggaccacttccagtaacaacaccgttggacccaccggacagacctgttgcatac5760attcctgagaactcgtgtgatcctcgagcggctatccgtggtgttgatgacagccaaggg5820aaatggttaggtggtatgtttgataaagacagctttgtggaaacatttgaaggttgggct5880aagacagtggttactggcagagcaaagcttggtggaattccagtgggtgtgatagctgtg5940gagactcagaccatgatgcaaactatccctgctgaccctggtcagcttgattcccgtgag6000caatctgttcctcgtgctggacaagtggggtttccagattctgcaaccaagactgcgcag6060gcattgctggacttcaaccgtgaaggattacctctgttcatcctcgctaactggagaggc6120ttctctggtggacaaagagatctttttgaaggaattcttcaggctggctcgactattgtt6180gagaaccttaggacatacaatcagcctgcctttgtctacattcccatggctgcagagcta6240cgaggaggggcttgggttgtggttgatagcaagataaacccagaccgcattgagtgctat6300gctgagaggactgcaaaaggcaatgttctggaaccgcaagggttaattgagatcaagttc6360aggtcagaggaactccaggattgcatgagtcggcttgacccaacattaattgatctgaaa6420gcaaaactcgaagtagcaaataaaaatggaagtgctgacacaaaatcgcttcaagaaaat6480atagaagctcgaacaaaacagttgatgcctctatatactcagattgcgatacggtttgct6540gaattgcatgatacatccctcagaatggctgcgaaaggtgtgattaagaaagttgtggac6600tgggaagaatcacgatctttcttctataagagattacggaggaggatctctgaggatgtt6660cttgcaaaagaaattagagctgtagcaggtgagcagttttcccaccaaccagcaatcgag6720ctgatcaagaaatggtattcagcttcacatgcagctgaatgggatgatgacgatgctttt6780gttgcttggatggataaccctgaaaactacaaggattatattcaatatcttaaggctcaa6840agagtatcccaatccctctcaagtctttcagattccagctcagatttgcaagccctgcca6900cagggtctttccatgttactagataagatggatccctctagaagagctcaacttgttgaa6960gaaatcaggaaggtccttggttga6984210102112327212PRT213水稻Oryzasativa嘉花1号ACCase-6028突变氨基酸序列40010MetThrSerThrHisValAlaThrLeuGlyValGlyAlaGlnAlaPro151015ProArgHisGlnLysLysSerAlaGlyThrAlaPheValSerSerGly202530SerSerArgProSerTyrArgLysAsnGlyGlnArgThrArgSerLeu354045ArgGluGluSerAsnGlyGlyValSerAspSerLysLysLeuAsnHis505560SerIleArgGlnGlyLeuAlaGlyIleIleAspLeuProAsnAspAla65707580AlaSerGluValAspIleSerHisGlySerGluAspProArgGlyPro859095ThrValProGlySerTyrGlnMetAsnGlyIleIleAsnGluThrHis100105110AsnGlyArgHisAlaSerValSerLysValValGluPheCysThrAla115120125LeuGlyGlyLysThrProIleHisSerValLeuValAlaAsnAsnGly130135140MetAlaAlaAlaLysPheMetArgSerValArgThrTrpAlaAsnAsp145150155160ThrPheGlySerGluLysAlaIleGlnLeuIleAlaMetAlaThrPro165170175GluAspLeuArgIleAsnAlaGluHisIleArgIleAlaAspGlnPhe180185190ValGluValProGlyGlyThrAsnAsnAsnAsnTyrAlaAsnValGln195200205LeuIleValGluIleAlaGluArgThrGlyValSerAlaValTrpPro210215220GlyTrpGlyHisAlaSerGluAsnProGluLeuProAspAlaLeuThr225230235240AlaLysGlyIleValPheLeuGlyProProAlaSerSerMetHisAla245250255LeuGlyAspLysValGlySerAlaLeuIleAlaGlnAlaAlaGlyVal260265270ProThrLeuAlaTrpSerGlySerHisValGluValProLeuGluCys275280285CysLeuAspSerIleProAspGluMetTyrArgLysAlaCysValThr290295300ThrThrGluGluAlaValAlaSerCysGlnValValGlyTyrProAla305310315320MetIleLysAlaSerTrpGlyGlyGlyGlyLysGlyIleArgLysVal325330335HisAsnAspAspGluValArgThrLeuPheLysGlnValGlnGlyGlu340345350ValProGlySerProIlePheIleMetArgLeuAlaAlaGlnSerArg355360365HisLeuGluValGlnLeuLeuCysAspGlnTyrGlyAsnValAlaAla370375380LeuHisSerArgAspCysSerValGlnArgArgHisGlnLysIleIle385390395400GluGluGlyProValThrValAlaProArgGluThrValLysGluLeu405410415GluGlnAlaAlaArgArgLeuAlaLysAlaValGlyTyrValGlyAla420425430AlaThrValGluTyrLeuTyrSerMetGluThrGlyGluTyrTyrPhe435440445LeuGluLeuAsnProArgLeuGlnValGluHisProValThrGluTrp450455460IleAlaGluValAsnLeuProAlaAlaGlnValAlaValGlyMetGly465470475480IleProLeuTrpGlnIleProGluIleArgArgPheTyrGlyMetAsn485490495HisGlyGlyGlyTyrAspLeuTrpArgLysThrAlaAlaLeuAlaThr500505510ProPheAsnPheAspGluValAspSerLysTrpProLysGlyHisCys515520525ValAlaValArgIleThrSerGluAspProAspAspGlyPheLysPro530535540ThrGlyGlyLysValLysGluIleSerPheLysSerLysProAsnVal545550555560TrpAlaTyrPheSerValLysSerGlyGlyGlyIleHisGluPheAla565570575AspSerGlnPheGlyHisValPheAlaTyrGlyThrThrArgSerAla580585590AlaIleThrThrMetAlaLeuAlaLeuLysGluValGlnIleArgGly595600605GluIleHisSerAsnValAspTyrThrValAspLeuLeuAsnAlaSer610615620AspPheArgGluAsnLysIleHisThrGlyTrpLeuAspThrArgIle625630635640AlaMetArgValGlnAlaGluArgProProTrpTyrIleSerValVal645650655GlyGlyAlaLeuTyrLysThrValThrAlaAsnThrAlaThrValSer660665670AspTyrValGlyTyrLeuThrLysGlyGlnIleProProLysHisIle675680685SerLeuValTyrThrThrValAlaLeuAsnIleAspGlyLysLysTyr690695700ThrIleAspThrValArgSerGlyHisGlySerTyrArgLeuArgMet705710715720AsnGlySerThrValAspAlaAsnValGlnIleLeuCysAspGlyGly725730735LeuLeuMetGlnLeuAspGlyAsnSerHisValIleTyrAlaGluGlu740745750GluAlaSerGlyThrArgLeuLeuIleAspGlyLysThrCysMetLeu755760765GlnAsnAspHisAspProSerLysLeuLeuAlaGluThrProCysLys770775780LeuLeuArgPheLeuValAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerAlaAlaIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleGlnArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyValValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValGlyPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGluGlyIleLeuGlnAlaGlySerThrIleValGluAsnLeuArg205020552060ThrTyrAsnGlnProAlaPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaLysGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly2325210112116984212DNA213水稻Oryzasativa嘉花1号ACCase-6030突变核苷酸序列40011atgacatccacacatgtggcgacattgggagttggtgcccaggcacctcctcgtcaccag60aaaaagtcagctggcactgcatttgtatcatctgggtcatcaagaccctcataccgaaag120aatggtcagcgtactcggtcacttagggaagaaagcaatggaggagtgtctgattccaaa180aagcttaaccactctattcgccaaggtcttgctggcatcattgacctcccaaatgacgca240gcttcagaagttgatatttcacatggttccgaagatcccagggggcctacggtcccaggt300tcctaccaaatgaatgggattatcaatgaaacacataatgggaggcatgcttcagtctcc360aaggttgttgagttttgtacggcacttggtggcaaaacaccaattcacagtgtattagtg420gccaacaatggaatggcagcagctaagttcatgcggagtgtccgaacatgggctaatgat480acttttggatcagagaaggcaattcagctgatagctatggcaactccggaggatctgagg540ataaatgcagagcacatcagaattgccgatcaatttgtagaggtacctggtggaacaaac600aacaacaactatgcaaatgtccaactcatagtggagatagcagagagaacaggtgtttct660gctgtttggcctggttggggtcatgcatctgagaatcctgaacttccagatgcgctgact720gcaaaaggaattgtttttcttgggccaccagcatcatcaatgcatgcattaggagacaag780gttggctcagctctcattgctcaagcagctggagttccaacacttgcttggagtggatca840catgtggaagttcctctggagtgttgcttggactcaatacctgatgagatgtatagaaaa900gcttgtgttactaccacagaggaagcagttgcaagttgtcaggtggttggttatcctgcc960atgattaaggcatcttggggtggtggtggtaaaggaataaggaaggttcataatgatgat1020gaggttaggacattatttaagcaagttcaaggcgaagtacctggttccccaatatttatc1080atgaggctagctgctcagagtcgacatcttgaagttcagttgctttgtgatcaatatggc1140aacgtagcagcacttcacagtcgagattgcagtgtacaacggcgacaccaaaagataatc1200gaggaaggaccagttactgttgctcctcgtgagactgtgaaagagcttgagcaggcagca1260cggaggcttgctaaagctgtgggttatgttggtgctgctactgttgaatacctttacagc1320atggaaactggtgaatattattttctggaacttaatccacggctacaggttgagcatcct1380gtcactgagtggatagctgaagtaaatttgcctgcggctcaagttgctgttggaatgggt1440atacccctttggcagattccagagatcaggcgcttctacggaatgaaccatggaggaggc1500tatgacctttggaggaaaacagcagctctagcgactccatttaactttgatgaagtagat1560tctaaatggccaaaaggccactgcgtagctgttagaataactagcgaggatccagatgat1620gggtttaagcctactggtggaaaagtaaaggagataagtttcaagagtaaaccaaatgtt1680tgggcctatttctcagtaaagtctggtggaggcatccatgaattcgctgattctcagttc1740ggacatgtttttgcgtatggaactactagatcggcagcaataactaccatggctcttgca1800ctaaaagaggttcaaattcgtggagaaattcattcaaacgtagactacacagttgaccta1860ttaaatgcctcagattttagagaaaataagattcatactggttggctggataccaggata1920gccatgcgtgttcaagctgagaggcctccatggtatatttcagtcgttggaggggcttta1980tataaaacagtaactgccaacacggccactgtttctgattatgttggttatcttaccaag2040ggccagattccaccaaagcatatatcccttgtctatacgactgttgctttgaatatagat2100gggaaaaaatatacaatcgatactgtgaggagtggacatggtagctacagattgcgaatg2160aatggatcaacggttgacgcaaatgtacaaatattatgtgatggtgggcttttaatgcag2220ctggatggaaacagccatgtaatttatgctgaagaagaggccagtggtacacgacttctt2280attgatggaaagacatgcatgttacagaatgaccatgacccatcaaagttattagctgag2340acaccatgcaaacttcttcgtttcttggttgctgatggtgctcatgttgatgctgatgta2400ccatatgcggaagttgaggttatgaagatgtgcatgcccctcttatcacccgcttctggt2460gtcatacatgttgtaatgtctgagggccaagcaatgcaggctggtgatcttatagctagg2520ct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GlyGlyValSerAspSerLysLysLeuAsnHis505560SerIleArgGlnGlyLeuAlaGlyIleIleAspLeuProAsnAspAla65707580AlaSerGluValAspIleSerHisGlySerGluAspProArgGlyPro859095ThrValProGlySerTyrGlnMetAsnGlyIleIleAsnGluThrHis100105110AsnGlyArgHisAlaSerValSerLysValValGluPheCysThrAla115120125LeuGlyGlyLysThrProIleHisSerValLeuValAlaAsnAsnGly130135140MetAlaAlaAlaLysPheMetArgSerValArgThrTrpAlaAsnAsp145150155160ThrPheGlySerGluLysAlaIleGlnLeuIleAlaMetAlaThrPro165170175GluAspLeuArgIleAsnAlaGluHisIleArgIleAlaAspGlnPhe180185190ValGluValProGlyGlyThrAsnAsnAsnAsnTyrAlaAsnValGln195200205LeuIleValGluIleAlaGluArgThrGlyValSerAlaValTrpPro210215220GlyTrpGlyHisAlaSerGluAsnProGluLeuProAspAlaLeuThr225230235240AlaLysGlyIleValPheLeuGlyProProAlaSerSerMetHisAla245250255LeuGlyAspLysValGlySerAlaLeuIleAlaGlnAlaAlaGlyVal260265270ProThrLeuAlaTrpSerGlySerHisValGluValProLeuGluCys275280285CysLeuAspSerIleProAspGluMetTyrArgLysAlaCysValThr290295300ThrThrGluGluAlaValAlaSerCysGlnValValGlyTyrProAla305310315320MetIleLysAlaSerTrpGlyGlyGlyGlyLysGlyIleArgLysVal325330335HisAsnAspAspGluValArgThrLeuPheLysGlnValGlnGlyGlu340345350ValProGlySerProIlePheIleMetArgLeuAlaAlaGlnSerArg355360365HisLeuGluValGlnLeuLeuCysAspGlnTyrGlyAsnValAlaAla370375380LeuHisSerArgAspCysSerValGlnArgArgHisGlnLysIleIle385390395400GluGluGlyProValThrValAlaProArgGluThrValLysGluLeu405410415GluGlnAlaAlaArgArgLeuAlaLysAlaValGlyTyrValGlyAla420425430AlaThrValGluTyrLeuTyrSerMetGluThrGlyGluTyrTyrPhe435440445LeuGluLeuAsnProArgLeuGlnValGluHisProValThrGluTrp450455460IleAlaGluValAsnLeuProAlaAlaGlnValAlaValGlyMetGly465470475480IleProLeuTrpGlnIleProGluIleArgArgPheTyrGlyMetAsn485490495HisGlyGlyGlyTyrAspLeuTrpArgLysThrAlaAlaLeuAlaThr500505510ProPheAsnPheAspGluValAspSerLysTrpProLysGlyHisCys515520525ValAlaValArgIleThrSerGluAspProAspAspGlyPheLysPro530535540ThrGlyGlyLysValLysGluIleSerPheLysSerLysProAsnVal545550555560TrpAlaTyrPheSerValLysSerGlyGlyGlyIleHisGluPheAla565570575AspSerGlnPheGlyHisValPheAlaTyrGlyThrThrArgSerAla580585590AlaIleThrThrMetAlaLeuAlaLeuLysGluValGlnIleArgGly595600605GluIleHisSerAsnValAspTyrThrValAspLeuLeuAsnAlaSer610615620AspPheArgGluAsnLysIleHisThrGlyTrpLeuAspThrArgIle625630635640AlaMetArgValGlnAlaGluArgProProTrpTyrIleSerValVal645650655GlyGlyAlaLeuTyrLysThrValThrAlaAsnThrAlaThrValSer660665670AspTyrValGlyTyrLeuThrLysGlyGlnIleProProLysHisIle675680685SerLeuValTyrThrThrValAlaLeuAsnIleAspGlyLysLysTyr690695700ThrIleAspThrValArgSerGlyHisGlySerTyrArgLeuArgMet705710715720AsnGlySerThrValAspAlaAsnValGlnIleLeuCysAspGlyGly725730735LeuLeuMetGlnLeuAspGlyAsnSerHisValIleTyrAlaGluGlu740745750GluAlaSerGlyThrArgLeuLeuIleAspGlyLysThrCysMetLeu755760765GlnAsnAspHisAspProSerLysLeuLeuAlaGluThrProCysLys770775780LeuLeuArgPheLeuValAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerAlaAlaIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleGlnArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyValValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValTrpPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGluGlyIleLeuGlnAlaGlySerThrIleValGluAsnLeuArg205020552060ThrTyrAsnGlnProAlaPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaLysGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly2325210132116984212DNA213水稻Oryzasativa嘉花1号ACCase-6149突变核苷酸序列40013atgacatccacacatgtggcgacattgggagttggtgcccaggcacctcctcgtcaccag60aaaaagtcagctggcactgcatttgtatcatctgggtcatcaagaccctcataccgaaag120aatggtcagcgtactcggtcacttagggaagaaagcaatggaggagtgtctgattccaaa180aagcttaaccactctattcgccaaggtcttgctggcatcattgacctcccaaatgacgca240gcttcagaagttgatatttcacatggttccgaagatcccagggggcctacggtcccaggt300tcctaccaaatgaatgggattatcaatgaaacacataatgggaggcatgcttcagtctcc360aaggttgttgagttttgtacggcacttggtggcaaaacaccaattcacagtgtattagtg420gccaacaatggaatggcagcagctaagttcatgcggagtgtccgaacatgggctaatgat480acttttggatcagagaaggcaattcagctgatagctatggcaactccggaggatctgagg540ataaatgcagagcacatcagaattgccgatcaatttgtagaggtacctggtggaacaaac600aacaacaactatgcaaatgtccaactcatagtggagatagcagagagaacaggtgtttct660gctgtttggcctggttggggtcatgcatctgagaatcctgaacttccagatgcgctgact720gcaaaaggaattgtttttcttgggccaccagcatcatcaatgcatgcattaggagacaag780gttggctcagctctcattgctcaagcagctggagttccaacacttgcttggagtggatca840catgtggaagttcctctggagtgttgcttggactcaatacctgatgagatgtatagaaaa900gcttgtgttactaccacagaggaagcagttgcaagttgtcaggtggttggttatcctgcc960atgattaaggcatcttggggtggtggtggtaaaggaataaggaaggttcataatgatgat1020gaggttaggacattatttaagcaagttcaaggcgaagtacctggttccccaatatttatc1080atgaggctagctgctcagagtcgacatcttgaagttcagttgctttgtgatcaatatggc1140aacgtagcagcacttcacagtcgagattgcagtgtacaacggcgacaccaaaagataatc1200gaggaaggaccagttactgttgctcctcgtgagactgtgaaagagcttgagcaggcagca1260cggaggcttgctaaagctgtgggttatgttggtgctgctactgttgaatacctttacagc1320atggaaactggtgaatattattttctggaacttaatccacggctacaggttgagcatcct1380gtcactgagtggatagctgaagtaaatttgcctgcggc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ctaactggagaggc6120ttctctggtggacaaagagatctttttgcaggaattcttcaggctggctcgactattgtt6180gagaaccttaggacatacaatcagcctgcctttgtctacattcccatggctgcagagcta6240cgaggaggggcttgggttgtggttgatagcaagataaacccagaccgcattgagtgctat6300gctgagaggactgcaaaaggcaatgttctggaaccgcaagggttaattgagatcaagttc6360aggtcagaggaactccaggattgcatgagtcggcttgacccaacattaattgatctgaaa6420gcaaaactcgaagtagcaaataaaaatggaagtgctgacacaaaatcgcttcaagaaaat6480atagaagctcgaacaaaacagttgatgcctctatatactcagattgcgatacggtttgct6540gaattgcatgatacatccctcagaatggctgcgaaaggtgtgattaagaaagttgtggac6600tgggaagaatcacgatctttcttctataagagattacggaggaggatctctgaggatgtt6660cttgcaaaagaaattagagctgtagcaggtgagcagttttcccaccaaccagcaatcgag6720ctgatcaagaaatggtattcagcttcacatgcagctgaatgggatgatgacgatgctttt6780gttgcttggatggataaccctgaaaactacaaggattatattcaatatcttaaggctcaa6840agagtatcccaatccctctcaagtctttcagattccagctcagatttgcaagccctgcca6900cagggtctttccatgttactagataagatggatccctctagaagagctcaacttgttgaa6960gaaatcaggaaggtccttggttga6984210142112327212PRT213水稻Oryzasativa嘉花1号ACCase-6149突变氨基酸序列40014MetThrSerThrHisValAlaThrLeuGlyValGlyAlaGlnAlaPro151015ProArgHisGlnLysLysSerAlaGlyThrAlaPheValSerSerGly202530SerSerArgProSerTyrArgLysAsnGlyGlnArgThrArgSerLeu354045ArgGluGluSerAsnGlyGlyValSerAspSerLysLysLeuAsnHis505560SerIleArgGlnGlyLeuAlaGlyIleIleAspLeuProAsnAspAla65707580AlaSerGluValAspIleSerHisGlySerGluAspProArgGlyPro859095ThrValProGlySerTyrGlnMetAsnGlyIleIleAsnGluThrHis100105110AsnGlyArgHisAlaSerValSerLysValValGluPheCysThrAla115120125LeuGlyGlyLysThrProIleHisSerValLeuValAlaAsnAsnGly130135140MetAlaAlaAlaLysPheMetArgSerValArgThrTrpAlaAsnAsp145150155160ThrPheGlySerGluLysAlaIleGlnLeuIleAlaMetAlaThrPro165170175GluAspLeuArgIleAsnAlaGluHisIleArgIleAlaAspGlnPhe180185190ValGluValProGlyGlyThrAsnAsnAsnAsnTyrAlaAsnValGln195200205LeuIleValGluIleAlaGluArgThrGlyValSerAlaValTrpPro210215220GlyTrpGlyHisAlaSerGluAsnProGluLeuProAspAlaLeuThr225230235240AlaLysGlyIleValPheLeuGlyProProAlaSerSerMetHisAla245250255LeuGlyAspLysValGlySerAlaLeuIleAlaGlnAlaAlaGlyVal260265270ProThrLeuAlaTrpSerGlySerHisValGluValProLeuGluCys275280285CysLeuAspSerIleProAspGluMetTyrArgLysAlaCysValThr290295300ThrThrGluGluAlaValAlaSerCysGlnValValGlyTyrProAla305310315320MetIleLysAlaSerTrpGlyGlyGlyGlyLysGlyIleArgLysVal325330335HisAsnAspAspGluValArgThrLeuPheLysGlnValGlnGlyGlu340345350ValProGlySerProIlePheIleMetArgLeuAlaAlaGlnSerArg355360365HisLeuGluValGlnLeuLeuCysAspGlnTyrGlyAsnValAlaAla370375380LeuHisSerArgAspCysSerValGlnArgArgHisGlnLysIleIle385390395400GluGluGlyProValThrValAlaProArgGluThrValLysGluLeu405410415GluGlnAlaAlaArgArgLeuAlaLysAlaValGlyTyrValGlyAla420425430AlaThrValGluTyrLeuTyrSerMetGluThrGlyGluTyrTyrPhe435440445LeuGluLeuAsnProArgLeuGlnValGluHisProValThrGluTrp450455460IleAlaGluValAsnLeuProAlaAlaGlnValAlaValGlyMetGly465470475480IleProLeuTrpGlnIleProGluIleArgArgPheTyrGlyMetAsn485490495HisGlyGlyGlyTyrAspLeuTrpArgLysThrAlaAlaLeuAlaThr500505510ProPheAsnPheAspGluValAspSerLysTrpProLysGlyHisCys515520525ValAlaValArgIleThrSerGluAspProAspAspGlyPheLysPro530535540ThrGlyGlyLysValLysGluIleSerPheLysSerLysProAsnVal545550555560TrpAlaTyrPheSerValLysSerGlyGlyGlyIleHisGluPheAla565570575AspSerGlnPheGlyHisValPheAlaTyrGlyThrThrArgSerAla580585590AlaIleThrThrMetAlaLeuAlaLeuLysGluValGlnIleArgGly595600605GluIleHisSerAsnValAspTyrThrValAspLeuLeuAsnAlaSer610615620AspPheArgGluAsnLysIleHisThrGlyTrpLeuAspThrArgIle625630635640AlaMetArgValGlnAlaGluArgProProTrpTyrIleSerValVal645650655GlyGlyAlaLeuTyrLysThrValThrAlaAsnThrAlaThrValSer660665670AspTyrValGlyTyrLeuThrLysGlyGlnIleProProLysHisIle675680685SerLeuValTyrThrThrValAlaLeuAsnIleAspGlyLysLysTyr690695700ThrIleAspThrValArgSerGlyHisGlySerTyrArgLeuArgMet705710715720AsnGlySerThrValAspAlaAsnValGlnIleLeuCysAspGlyGly725730735LeuLeuMetGlnLeuAspGlyAsnSerHisValIleTyrAlaGluGlu740745750GluAlaSerGlyThrArgLeuLeuIleAspGlyLysThrCysMetLeu755760765GlnAsnAspHisAspProSerLysLeuLeuAlaGluThrProCysLys770775780LeuLeuArgPheLeuValAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerAlaAlaIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleGlnArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyValValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValTrpPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGlyGlyIleLeuGlnAlaGlySerThrIleValGluAsnLeuArg205020552060ThrTyrAsnGlnProAlaPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaLysGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly2325210152116984212DNA213水稻Oryzasativa嘉花1号ACCase-6178突变核苷酸序列40015atgacatccacacatgtggcgacattgggagttggtgcccaggcacctcctcgtcaccag60aaaaagtcagctggcactgcatttgtatcatctgggtcatcaagaccctcataccgaaag120aatggtcagcgtactcggtcacttagggaagaaagcaatggaggagtgtctgattccaaa180aagcttaaccactctattcgccaaggtcttgctggcatcattgacctcccaaatgacgca240gcttcagaagttgatatttcacatggttccgaagatcccagggggcctacggtcccaggt30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alAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerAlaAlaIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleGlnArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyValValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValTrpPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGluGlyIleLeuGlnAlaGlySerThrIleLeuGluAsnLeuArg205020552060ThrTyrAsnGlnProAlaPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaLysGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly2325210172116984212DNA213水稻Oryzasativa嘉花1号ACCase-6209突变核苷酸序列40017atgacatccacacatgtggcgacattgggagttggtgcccaggcacctcctcgtcaccag60aaaaagtcagctggcactgcatttgtatcatctgggtcatcaagaccctcataccgaaag120aatggtcagcgtactcggtcacttagggaagaaagcaatggaggagtgtctgattccaaa180aagcttaaccactctattcgccaaggtcttgctggcatcattgacctcccaaatgacgca240gcttcagaagttgatatttcacatggttccgaagatcccagggggcctacggtcccaggt300tcctaccaaatgaatgggattatcaatgaaacacataatgggaggcatgcttcagtctcc360aaggttgttgagttttgtacggcacttggtggcaaaacaccaattcacagtgtattagtg420gccaacaatggaatggcagcagctaagttcatgcggagtgtccgaacatgggctaatgat480acttttggatcagagaaggcaattcagctgatagctatggcaactccggaggatctgagg540ataaatgcagagcacatcagaattgccgatcaatttgtagaggtacctggtggaacaaac600aacaacaactatgcaaatgtccaactcatagtggagatagcagagagaacaggtgtttct660gctgtttggcctggttggggtcatgcatctgagaatcctgaacttccagatgcgctgact720gcaaaaggaattgtttttcttgggccaccagcatcatcaatgcatgcattaggagacaag780gttggctcagctctcattgctcaagcagctggagttccaacacttgcttggagtggatca840catgtggaagttcctctggagtgttgcttggactcaatacctgatgagatgtatagaaaa900gcttgtgttactaccacagaggaagcagttgcaagttgtcaggtggttggttatcctgcc960atgattaaggcatcttggggtggtggtggtaaaggaataaggaaggttcataatgatgat1020gaggttaggacattatttaagcaagttcaaggcgaagtacctggttccccaatatttatc1080atgaggctagctgctcagagtcgacatcttgaagttcagttgctttgtgatcaatatggc1140aacgtagcagcacttcacagtcgagattgcagtgtacaacggcgacaccaaaagataatc1200gaggaaggaccagttactgttgctcctcgtgagactgtgaaagagcttgagcaggcagca1260cggaggcttgctaaagctgtgggttatgttggtgctgctactgttgaatacctttacagc1320atggaaactggtgaatattattttctggaacttaatccacggctacaggttgagcatcct1380gtcactgagtggatagctgaagtaaatttgcctgcggctcaagttgctgttggaatgggt1440atacccctttggcagattccagagatcaggcgcttctacggaatgaaccatggaggaggc1500tatgacctttggaggaaaacagcagctctagcgactccatttaactttgatgaagtagat1560tctaaatggccaaaaggccactgcgtagctgttagaataactagcgaggatccagatgat1620gggtttaagcctactggtggaaaagtaaaggagataagtttcaagagtaaaccaaatgtt1680tgggcctatttctcagtaaagtctggtggaggcatccatgaattcgctgattctcagttc1740ggacatgtttttgcgtatggaactactagatcggcagcaataactaccatggctcttgca1800ctaaaagaggttcaaattcgtggagaaattcattcaaacgtagactacacagttgaccta1860ttaaatgcctcagattttagagaaaataagattcatactggttggctggataccaggata1920gccatgcgtgttcaagctgagaggcctccatggtatatttcagtcgttggaggggcttta1980tataaaacagtaactgccaacacggccactgtttctgattatgttggttatcttaccaag2040ggccagattccaccaaagcatatatcccttgtctatacgactgttgctttgaatatagat2100gggaaaaaatatacaatcgatactgtgaggagtggacatggtagctacagattgcgaatg2160aatggatcaacggttgacgcaaatgtacaaatattatgtgatggtgggcttttaatgcag2220ctggatggaaacagccatgtaatttatgctgaagaagaggccagtggtacacgacttctt2280attgatggaaagacatgcatgttacagaatgaccatgacccatcaaagttattagctgag2340acaccatgcaaacttcttcgtttcttggttgctgatggtgctcatgttgatgctgatgta2400ccatatgcggaagttgaggttatgaagatgtgcatgcccctcttatcacccgcttctggt2460gtcatacatgttgtaatgtctgagggccaagcaatgcaggctggtgatcttatagctagg2520ctggatcttgatgacccttctgctgttaagagagctgagccgttcgaagatacttttcca2580caaatgggtctccctattgctgcttctggccaagttcacaaattatgtgctgcaagtctg2640aatgcttgtcgaatgatccttgcggggtatgagcatgatattgacaaggttgtgccagag2700ttggtatactgcctagacactccggagcttcctttcctgcagtgggaggagcttatgtct2760gttttagcaactagacttccaagaaatcttaaaagtgagttggagggcaaatatgaggaa2820tacaaagtaaaatttgactctgggataatcaatgatttccctgccaatatgctacgagtg2880ataattgaggaaaatcttgcatgtggttctgagaaggagaaggctacaaatgagaggctt2940gttgagcctcttatgagcctactgaagtcatatgagggtgggagagaaagtcatgctcac3000tttgttgtcaagtccctttttgaggagtatctctatgttgaagaattgttcagtgatgga3060attcagtctgatgtgattgagcgtctgcgccttcaacatagtaaagacctacagaaggtc3120gtagacattgtgttgtcccaccagagtgttagaaataaaactaagctgatactaaaactc3180atggagagtctggtctatccaaatcctgctgcctacagggatcaattgattcgcttttct3240tcccttaatcacaaagcgtattacaagttggcacttaaagctagtgaacttcttgaacaa3300acaaaacttagtgagctccgtgcaagaatagcaaggagcctttcagagctggagatgttt3360actgaggaaagcaagggtctctccatgcataagcgagaaattgccattaaggagagcatg3420gaagatttagtcactgctccactgccagttgaagatgcgctcatttctttatttgattgt3480agtgatacaactgttcaacagagagtgattgagacttatatagctcgattataccagcct3540catcttgtaaaggacagtatcaaaatgaaatggatagaatcgggtgttattgctttatgg3600gaatttcctgaagggcattttgatgcaagaaatggaggagcggttcttggtgacaaaaga3660tggggtgccatggtcattgtcaagtctcttgaatcactttcaatggccattagatttgca3720ctaaaggagacatcacactacactagctctgagggcaatatgatgcatattgctttgttg3780ggtgctgataataagatgcatataattcaagaaagtggtgatgatgctgacagaatagcc3840aaacttcccttgatactaaaggataatgtaaccgatctgcatgcctctggtgtg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Trp450455460IleAlaGluValAsnLeuProAlaAlaGlnValAlaValGlyMetGly465470475480IleProLeuTrpGlnIleProGluIleArgArgPheTyrGlyMetAsn485490495HisGlyGlyGlyTyrAspLeuTrpArgLysThrAlaAlaLeuAlaThr500505510ProPheAsnPheAspGluValAspSerLysTrpProLysGlyHisCys515520525ValAlaValArgIleThrSerGluAspProAspAspGlyPheLysPro530535540ThrGlyGlyLysValLysGluIleSerPheLysSerLysProAsnVal545550555560TrpAlaTyrPheSerValLysSerGlyGlyGlyIleHisGluPheAla565570575AspSerGlnPheGlyHisValPheAlaTyrGlyThrThrArgSerAla580585590AlaIleThrThrMetAlaLeuAlaLeuLysGluValGlnIleArgGly595600605GluIleHisSerAsnValAspTyrThrValAspLeuLeuAsnAlaSer610615620AspPheArgGluAsnLysIleHisThrGlyTrpLeuAspThrArgIle625630635640AlaMetArgValGlnAlaGluArgProProTrpTyrIleSerValVal645650655GlyGlyAlaLeuTyrLysThrValThrAlaAsnThrAlaThrValSer660665670AspTyrValGlyTyrLeuThrLysGlyGlnIleProProLysHisIle675680685SerLeuValTyrThrThrValAlaLeuAsnIleAspGlyLysLysTyr690695700ThrIleAspThrValArgSerGlyHisGlySerTyrArgLeuArgMet705710715720AsnGlySerThrValAspAlaAsnValGlnIleLeuCysAspGlyGly725730735LeuLeuMetGlnLeuAspGlyAsnSerHisValIleTyrAlaGluGlu740745750GluAlaSerGlyThrArgLeuLeuIleAspGlyLysThrCysMetLeu755760765GlnAsnAspHisAspProSerLysLeuLeuAlaGluThrProCysLys770775780LeuLeuArgPheLeuValAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerAlaAlaIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleGlnArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyValValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValTrpPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGluGlyIleLeuGlnAlaGlySerThrIleValGluAsnLeuArg205020552060ThrTyrAsnGlnProValPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaLysGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly2325210192116984212DNA213水稻Oryzasativa嘉花1号ACCase-6316突变核苷酸序列40019atgacatccacacatgtggcgacattgggagttggtgcccaggcacctcctcgtcaccag60aaaaagtcagctggcactgcatttgtatcatctgggtcatcaagaccctcataccgaaag120aatggtcagcgtactcggtcacttagggaagaaagcaatggaggagtgtctgattccaaa180aagcttaaccactctattcgccaaggtcttgctggcatcattgacctcccaaatgacgca240gcttcagaagttgatatttcacatggttccgaagatcccagggggcctacggtcccaggt300tcctaccaaatgaatgggattatcaatgaaacacataatgggaggcatgcttcagtctcc360aaggttgttgagttttgtacggcacttggtggcaaaacaccaattcacagtgtattagtg420gccaacaatggaatggcagcagctaagttcatgcggagtgtccgaacatgggctaatgat480acttttggatcagagaaggcaattcagctgatagctatggcaactccggaggatctgagg540ataaatgcagagcacatcagaattgccgatcaatttgtagaggtacctggtggaacaaac600aacaacaactatgcaaatgtccaactcatagtggagatagcagagagaacaggtgtttct660gctgtttggcctggttggggtcatgcatctgagaatcctgaacttccagatgcgctgact720gcaaaaggaattgtttttcttgggccaccagcatcatcaatgcatgcattaggagacaag780gttggctcagctctcattgctcaagcagctggagttccaacacttgcttggagtggatca840catgtggaagttcctctggagtgttgcttggactcaatacctgatgagatgtatagaaaa900gcttgtgttactaccacagaggaagcagttgcaagttgtcaggtggttggttatcctgcc960atgattaaggcatcttggggtggtggtggtaaaggaataaggaaggttcataatgatgat1020gaggttaggacattatttaagcaagttcaaggcgaagtacctggttccccaatatttatc1080atgaggctagctgctcagagtcgacatcttgaagttcagttgctttgtgatcaatatggc1140aacgtagcagcacttcacagtcgagattgcagtgtacaacggcgacaccaaaagataatc1200gaggaaggaccagttactgttgctcctcgtgagactgtgaaagagcttgagcaggcagca1260cggaggcttgctaaagctgtgggttatgttggtgctgctactgttgaatacctttacagc1320atggaaactggtgaatattattttctggaacttaatccacggctacaggttgagcatcct1380gtcactgagtggatagctgaagtaaatttgcctgcggctcaagttgctgttggaatgggt1440atacccctttggcagattccagagatcaggcgcttctacggaatgaaccatggaggaggc1500tatgacctttggaggaaaacagcagctctagcgactccatttaactttgatgaagtagat1560tctaaatggccaaaaggccactgcgtagctgttagaataactagcgaggatccagatgat1620gggtttaagcctactggtggaaaagtaaaggagataagtttcaagagtaaaccaaatgtt1680tgggcctatttctcagtaaagtctggtggaggcatccatgaattcgctgattctcagttc1740ggacatgtttttgcgtatggaactactagatcggcagcaataactaccatggctcttgca1800ctaaaagaggttcaaattcgtggagaaattcattcaaacgtagactacacagttgaccta1860ttaaatgcctcagattttagagaaaataagattcatactggttggctggataccaggata1920gccatgcgtgttcaagctgagaggcctccatggtatatttcagtcgttggaggggcttta1980tataaaacagtaactgccaacacggccactgtttctgattatgttggttatcttaccaag2040ggccagattccaccaaagcatatatcccttgtctatacgactgttgctttgaatatagat2100gggaaaaaatatacaatcgatactgtgaggagtggacatggtagctacagattgcgaatg2160aatggatcaacggttgacgcaaatgtacaaatattatgtgatggtgggcttttaatgcag2220ctggatggaaacagccatgtaatttatgctgaagaagaggccagtggtacacgacttctt2280attgatggaaagacatgcatgttacagaatgaccatgacccatcaaagttattagctgag2340acaccatgcaaacttcttcgtttcttggttgctgatggtgctcatgttgatgctgatgta2400ccatatgcggaagttgaggttatgaagatgtgcatgcccctcttatcacccgcttctggt2460gtcatacatgttgtaatgtctgagggccaagcaatgcaggctggtgatcttatagctagg2520ctggatcttgatgacccttctgctgttaagagagctgagccgttcgaagatacttttcca2580caaatgggtctccctattgctgcttctggccaagttcacaaattatgtgctgcaagtctg2640aatgcttgtcgaatgatccttgcggggtatgagcatgatattgacaaggttgtgccagag2700ttggtatactgcctagacactccggagcttcctttcctgcagtgggaggagcttatgtct2760gttttagcaactagacttccaagaaa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oIleHisSerValLeuValAlaAsnAsnGly130135140MetAlaAlaAlaLysPheMetArgSerValArgThrTrpAlaAsnAsp145150155160ThrPheGlySerGluLysAlaIleGlnLeuIleAlaMetAlaThrPro165170175GluAspLeuArgIleAsnAlaGluHisIleArgIleAlaAspGlnPhe180185190ValGluValProGlyGlyThrAsnAsnAsnAsnTyrAlaAsnValGln195200205LeuIleValGluIleAlaGluArgThrGlyValSerAlaValTrpPro210215220GlyTrpGlyHisAlaSerGluAsnProGluLeuProAspAlaLeuThr225230235240AlaLysGlyIleValPheLeuGlyProProAlaSerSerMetHisAla245250255LeuGlyAspLysValGlySerAlaLeuIleAlaGlnAlaAlaGlyVal260265270ProThrLeuAlaTrpSerGlySerHisValGluValProLeuGluCys275280285CysLeuAspSerIleProAspGluMetTyrArgLysAlaCysValThr290295300ThrThrGluGluAlaValAlaSerCysGlnValValGlyTyrProAla305310315320MetIleLysAlaSerTrpGlyGlyGlyGlyLysGlyIleArgLysVal325330335HisAsnAspAspGluValArgThrLeuPheLysGlnValGlnGlyGlu340345350ValProGlySerProIlePheIleMetArgLeuAlaAlaGlnSerArg355360365HisLeuGluValGlnLeuLeuCysAspGlnTyrGlyAsnValAlaAla370375380LeuHisSerArgAspCysSerValGlnArgArgHisGlnLysIleIle385390395400GluGluGlyProValThrValAlaProArgGluThrValLysGluLeu405410415GluGlnAlaAlaArgArgLeuAlaLysAlaValGlyTyrValGlyAla420425430AlaThrValGluTyrLeuTyrSerMetGluThrGlyGluTyrTyrPhe435440445LeuGluLeuAsnProArgLeuGlnValGluHisProValThrGluTrp450455460IleAlaGluValAsnLeuProAlaAlaGlnValAlaValGlyMetGly465470475480IleProLeuTrpGlnIleProGluIleArgArgPheTyrGlyMetAsn485490495HisGlyGlyGlyTyrAspLeuTrpArgLysThrAlaAlaLeuAlaThr500505510ProPheAsnPheAspGluValAspSerLysTrpProLysGlyHisCys515520525ValAlaValArgIleThrSerGluAspProAspAspGlyPheLysPro530535540ThrGlyGlyLysValLysGluIleSerPheLysSerLysProAsnVal545550555560TrpAlaTyrPheSerValLysSerGlyGlyGlyIleHisGluPheAla565570575AspSerGlnPheGlyHisValPheAlaTyrGlyThrThrArgSerAla580585590AlaIleThrThrMetAlaLeuAlaLeuLysGluValGlnIleArgGly595600605GluIleHisSerAsnValAspTyrThrValAspLeuLeuAsnAlaSer610615620AspPheArgGluAsnLysIleHisThrGlyTrpLeuAspThrArgIle625630635640AlaMetArgValGlnAlaGluArgProProTrpTyrIleSerValVal645650655GlyGlyAlaLeuTyrLysThrValThrAlaAsnThrAlaThrValSer660665670AspTyrValGlyTyrLeuThrLysGlyGlnIleProProLysHisIle675680685SerLeuValTyrThrThrValAlaLeuAsnIleAspGlyLysLysTyr690695700ThrIleAspThrValArgSerGlyHisGlySerTyrArgLeuArgMet705710715720AsnGlySerThrValAspAlaAsnValGlnIleLeuCysAspGlyGly725730735LeuLeuMetGlnLeuAspGlyAsnSerHisValIleTyrAlaGluGlu740745750GluAlaSerGlyThrArgLeuLeuIleAspGlyLysThrCysMetLeu755760765GlnAsnAspHisAspProSerLysLeuLeuAlaGluThrProCysLys770775780LeuLeuArgPheLeuValAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerAlaAlaIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleGlnArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyValValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValTrpPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGluGlyIleLeuGlnAlaGlySerThrIleValGluAsnLeuArg205020552060ThrTyrAsnGlnProAlaPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaGluGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly2325210212116984212DNA213水稻Oryzasativa嘉花1号ACCase野生型基因序列40021atgacatccacacatgtggcgacattgggagttggtgcccaggcacctcctcgtcaccag60aaaaagtcagctggcactgcatttgtatcatctgggtcatcaagaccctcataccgaaag120aatggtcagcgtactcggtcacttagggaagaaagcaatggaggagtgtctgattccaaa180aagcttaaccactctattcgccaaggtcttgctggcatcattgacctcccaaatgacgca240gcttcagaagttgatatttcacatggttccgaagatcccagggggcctacggtcccaggt300tcctaccaaatgaatgggattatcaatgaaacacataatgggaggcatgcttcagtctcc360aaggttgttgagttttgtacggcacttggtggcaaaacaccaattcacagtgtattagtg420gccaacaatggaatggcagcagctaagttcatgcggagtgtccgaacatgggctaatgat480acttttggatcagagaaggcaattcagctgatagctatggcaactccggaggatctgagg540ataaatgcagagcacatcagaattgccgatcaatttgtagaggtacctggtggaacaaac600aacaacaactatgcaaatgtccaactcatagtggagatagcagagagaacaggtgtttct660gctgtttggcctggttggggtcatgcatctgagaatcctgaacttccagatgcgctgact720gcaaaaggaattgtttttcttgggccaccagcatcatcaatgcatgcattaggagacaag780gttggctcagctctcattgctcaagcagctggagttccaacacttgcttggagtggatca840catgtggaagttcctctggagtgttgcttggactcaatacctgatgagatgtatagaaaa900gcttgtgttactaccacagaggaagcagttgcaagttgtcaggtggttggttatcctgcc960atgattaaggcatcttggggtggtggtggtaaaggaataaggaaggttcataatgatgat1020gaggttaggacattatttaagcaagttcaaggcgaagtacctggttccccaatatttatc1080atgaggctagctgctcagagtcgacatcttgaagttcagttgctttgtgatcaatatggc1140aacgtagcagcacttcacagtcgagattgcagtgtacaacggcgacaccaaaagataatc1200gaggaaggaccagttactgttgctcctcgtgagactgtgaaagagcttgagcaggcagca1260cggaggcttgctaaagctgtgggttatgttggtgctgctactgttgaatacctttacagc1320atggaaactggtgaatattattttctggaacttaatccacggctacaggttgagcatcct1380gtcactgagtggatagctgaagtaaatttgcctgcggctcaagttgctgttggaatgggt1440atacccctttggcagattccagagatcaggcgcttctacggaatgaaccatggaggaggc1500tatgacctttggaggaaaacagcagctctagcgactccatttaactttgatgaagtagat1560tctaaatggccaaaaggccactgcgtagctgttagaataactagcgaggatccagatgat1620gggtttaagcctactggtggaaaagtaaaggagataagtttcaagagtaaaccaaatgtt1680tgggcctatttctcagtaaagtctggtggaggcatccatgaattcgctgattctcagttc1740ggacatgtttttgcgtatggaactactagatcggcagcaataactaccatggctcttgca1800ctaaaagaggttcaaattcgtggagaaattcattcaaacgtagactacacagttgaccta1860ttaaatgcctcagattttagagaaaataagattcatactggttggctggataccaggata1920gccatgcgtgttcaagctgagaggcctccatggtatatttcagtcgttggaggggcttta1980tataaaacagtaactgccaacacggccactgtttctgattatgttggttatcttaccaag2040ggccagattccaccaaagcatatatcccttgtctatacgactgttgctttgaatatagat2100gggaaaaaatatacaatcgatactgtgaggagtggacatggtagctacagattgcgaatg2160aatggatcaacggttgacgcaaatgtacaaatattatgtgatggtgggcttttaatgcag2220ctggatggaaacagccatgtaatttatgctgaagaagaggccagtggtacacgacttctt2280attgatggaaagacatgcatgttacagaatgaccatgacccatcaaagttattagctgag2340acaccatgcaaacttcttcgtttcttggttgctgatggtgctcatgttgatgctgatgta2400ccatatgcggaagttgaggttatgaagatgtgcatgcccctcttatcacccgcttctggt2460gtcatacatgttgtaatgtctgagggccaagcaatgcaggctggtgatcttatagctagg2520ctggatcttgatgacccttctgctgttaagagagctgagccgttcgaagatacttttcca2580caaatgggtctccctattgctgcttctggccaagttcacaaattatgtgctgcaagtctg2640aatgcttgtcgaatgatccttgcggggtatgagcatgatattgacaaggttgtgccagag2700ttggtatactgcctagacactccggagcttcctttcctgcagtgggaggagcttatgtct2760gttttagcaactagacttccaagaaatcttaaaagtgagttggagggcaaatatgaggaa2820tacaaagtaaaatttgactctgggataatcaatgatttccctgccaatatgctacgagtg2880ataattgaggaaaatcttgcatgtggttctgagaaggagaaggctacaaatgagaggctt2940gttgagcctcttatgagcctactgaagtcatatgagggtgggagagaaagtcatgctcac3000tttgttgtcaagtccctttttgaggagtatctctatgttgaagaattgttcagtgatgga3060attcagtctgatgtgattgagcgtctgcgccttcaacatagtaaagacctacagaaggtc3120gtagacattgtgttgtcccaccagagtgttagaaataaaactaagctgatactaaaactc3180atggagagtctggtctatccaaatcctgctgcctacagggatcaattgattcgcttttct3240tcccttaatcacaaagcgtattacaagttggcacttaaagctagtgaacttcttgaacaa3300acaaaacttagtgagctccgtgcaagaatagcaaggagcctttcagagctggagatgttt3360actgaggaaagcaagggtctctccatgcataagcgagaaattgccattaaggagagcatg3420gaagatttagtcactgctccactgccagttgaagatgcgctcatttctttatttgattgt3480agtgatacaactgttcaacagagagtgattgagacttatatagctcgattataccagcct3540catcttgtaaaggacagtatcaaaatgaaatggatagaatcgggtgttattgctttatgg3600gaatttcctgaagggcattttgatgcaagaaatggaggagcggttcttggtgacaaaaga3660tggggtgccatggtcattgtcaagtctcttgaatcactttcaatggccattagatttgca3720ctaaaggagacatcacactacactagctctgagggcaatatgatgcatattgctttgttg3780ggtgctgataataagatgcatataattcaagaaagtggtgatgatgctgacagaatagcc3840aaacttcccttgatactaaaggataatgtaaccgatctgcatgcctctggtgtgaaaaca3900ataagtttcattgttcaaagagatgaagcacggatgacaatgcgtcgtaccttcctttgg3960tctgatgaaaagctttcttatgaggaagagccaattctccggcatgtggaacctcctctt4020tctgcacttcttgagttggacaagttgaaagtgaaaggatacaatgaaatgaagtatacc4080ccatcacgggatcgtcaatggcatatctacacacttagaaatactgaaaaccccaaaatg4140ttgcaccgggtatttttccgaacccttgtcaggcaacccagtgtatccaacaagttttct4200tcgggccagattggtgacatggaagttgggagtgctgaagaacctctgtcatttacatca4260accagcatattaagatctttgatgactgctatagaggaattggagcttcacgcaattaga4320actggccattcacacatgtatttgcatgtattgaaagaacaaaagcttcttgatcttgtt4380ccagtttcagggaatacagttttggatgttggtcaagatgaagctactgcatattcactt4440ttaaaagaaatggctatgaagatacatgaacttgttggtgcaagaatgcaccatctttct4500gtatgccaatgggaagtgaaacttaagttggactgcgatggtcctgccagtggtacctgg4560aggattgtaacaaccaatgttactagtcacacttgcactgtggatatctaccgtgagatg4620gaagataaagaatcacggaagttagtataccatcccgccactccggcggctggtcctctg4680catggtgtggcactgaataatccatatcagcctttgagtgtcattgatctcaaacgctgt4740tctgctaggaataatagaactacatactgctatgattttccactggcatttgaaactgca4800gtgaggaagtcatggtcctctagtacctctggtgcttctaaaggtgttgaaaatgcccaa4860tgttatgttaaagctacagagttggtatttgcggacaaacatgggtcatggggcactcct4920ttagttcaaatggaccggcctgctgggctcaatgacattggtatggtagcttggaccttg4980aagatgtccactcctgaatttcctagtggtagggagattattgttgttgcaaatgatatt5040acgttcagagctggatcatttggcccaagggaagatgcattttttgaagctgttaccaac5100ctagcctgtgagaagaaacttcctcttatttatttggcagcaaattctggtgctcgaatt5160ggcatagcagatgaagtgaaatcttgcttccgtgttgggtggtctgatgatggcagccct5220gaacgtgggtttcagtacatttatctaagcgaagaagactatgctcgtattggcacttct5280gtcatagcacataagatgcagctagacagtggtgaaattaggtgggttattgattctgtt5340gtgggcaaggaagatggacttggtgtggagaatatacatggaagtgctgctattgccagt5400gcttattctagggcatataaggagacatttacacttacatttgtgactggaagaactgtt5460ggaataggagcttatcttgctcgacttggcatccggtgcatacagcgtcttgaccagcct5520attattcttacaggctattctgcactgaacaagcttcttgggcgggaagtgtacagctcc5580cacatgcagttgggtggtcccaaaatcatggcaactaatggtgttgtccatcttactgtt5640tcagatgaccttgaaggcgtttctaatatattgaggtggctcagttatgttcctgcctac5700attggtggaccacttccagtaacaacaccgttggacccaccggacagacctgttgcatac5760attcctgagaactcgtgtgatcctcgagcggctatccgtggtgttgatgacagccaaggg5820aaatggttaggtggtatgtttgataaagacagctttgtggaaacatttgaaggttgggct5880aagacagtggttactggcagagcaaagcttggtggaattccagtgggtgtgatagctgtg5940gagactcagaccatgatgcaaactatccctgctgaccctggtcagcttgattcccgtgag6000caatctgttcctcgtgctggacaagtgtggtttccagattctgcaaccaagactgcgcag6060gcattgctggacttcaaccgtgaaggattacctctgttcatcctcgctaactggagaggc6120ttctctggtggacaaagagatctttttgaaggaattcttcaggctggctcgactattgtt6180gagaaccttaggacatacaatcagcctgcctttgtctacattcccatggctgcagagcta6240cgaggaggggcttgggttgtggttgatagcaagataaacccagaccgcattgagtgctat6300gctgagaggactgcaaaaggcaatgttctggaaccgcaagggttaattgagatcaagttc6360aggtcagaggaactccaggattgcatgagtcggcttgacccaacattaattgatctgaaa6420gcaaaactcgaagtagcaaataaaaatggaagtgctgacacaaaatcgcttcaagaaaat6480atagaagctcgaacaaaacagttgatgcctctatatactcagattgcgatacggtttgct6540gaattgcatgatacatccctcagaatggctgcgaaaggtgtgattaagaaagttgtggac6600tgggaagaatcacgatctttcttctataagagattacggaggaggatctctgaggatgtt6660cttgcaaaagaaattagagctgtagcaggtgagcagttttcccaccaaccagcaatcgag6720ctgatcaagaaatggtattcagcttcacatgcagctgaatgggatgatgacgatgctttt6780gttgcttggatggataaccctgaaaactacaaggattatattcaatatcttaaggctcaa6840agagtatcccaatccctctcaagtctttcagattccagctcagatttgcaagccctgcca6900cagggtctttccatgttactagataagatggatccctctagaagagctcaacttgttgaa6960gaaatcaggaaggtccttggttga6984210222112327212PRT213水稻Oryzasativa嘉花1号ACCase野生型氨基酸序列40022MetThrSerThrHisValAlaThrLeuGlyValGlyAlaGlnAlaPro151015ProArgHisGlnLysLysSerAlaGlyThrAlaPheValSerSerGly202530SerSerArgProSerTyrArgLysAsnGlyGlnArgThrArgSerLeu354045ArgGluGluSerAsnGlyGlyValSerAspSerLysLysLeuAsnHis505560SerIleArgGlnGlyLeuAlaGlyIleIleAspLeuProAsnAspAla65707580AlaSerGluValAspIleSerHisGlySerGluAspProArgGlyPro859095ThrValProGlySerTyrGlnMetAsnGlyIleIleAsnGluThrHis100105110AsnGlyArgHisAlaSerValSerLysValValGluPheCysThrAla115120125LeuGlyGlyLysThrProIleHisSerValLeuValAlaAsnAsnGly130135140MetAlaAlaAlaLysPheMetArgSerValArgThrTrpAlaAsnAsp145150155160ThrPheGlySerGluLysAlaIleGlnLeuIleAlaMetAlaThrPro165170175GluAspLeuArgIleAsnAlaGluHisIleArgIleAlaAspGlnPhe180185190ValGluValProGlyGlyThrAsnAsnAsnAsnTyrAlaAsnValGln195200205LeuIleValGluIleAlaGluArgThrGlyValSerAlaValTrpPro210215220GlyTrpGlyHisAlaSerGluAsnProGluLeuProAspAlaLeuThr225230235240AlaLysGlyIleValPheLeuGlyProProAlaSerSerMetHisAla245250255LeuGlyAspLysValGlySerAlaLeuIleAlaGlnAlaAlaGlyVal260265270ProThrLeuAlaTrpSerGlySerHisValGluValProLeuGluCys275280285CysLeuAspSerIleProAspGluMetTyrArgLysAlaCysValThr290295300ThrThrGluGluAlaValAlaSerCysGlnValValGlyTyrProAla305310315320MetIleLysAlaSerTrpGlyGlyGlyGlyLysGlyIleArgLysVal325330335HisAsnAspAspGluValArgThrLeuPheLysGlnValGlnGlyGlu340345350ValProGlySerProIlePheIleMetArgLeuAlaAlaGlnSerArg355360365HisLeuGluValGlnLeuLeuCysAspGlnTyrGlyAsnValAlaAla370375380LeuHisSerArgAspCysSerValGlnArgArgHisGlnLysIleIle385390395400GluGluGlyProValThrValAlaProArgGluThrValLysGluLeu405410415GluGlnAlaAlaArgArgLeuAlaLysAlaValGlyTyrValGlyAla420425430AlaThrValGluTyrLeuTyrSerMetGluThrGlyGluTyrTyrPhe435440445LeuGluLeuAsnProArgLeuGlnValGluHisProValThrGluTrp450455460IleAlaGluValAsnLeuProAlaAlaGlnValAlaValGlyMetGly465470475480IleProLeuTrpGlnIleProGluIleArgArgPheTyrGlyMetAsn485490495HisGlyGlyGlyTyrAspLeuTrpArgLysThrAlaAlaLeuAlaThr500505510ProPheAsnPheAspGluValAspSerLysTrpProLysGlyHisCys515520525ValAlaValArgIleThrSerGluAspProAspAspGlyPheLysPro530535540ThrGlyGlyLysValLysGluIleSerPheLysSerLysProAsnVal545550555560TrpAlaTyrPheSerValLysSerGlyGlyGlyIleHisGluPheAla565570575AspSerGlnPheGlyHisValPheAlaTyrGlyThrThrArgSerAla580585590AlaIleThrThrMetAlaLeuAlaLeuLysGluValGlnIleArgGly595600605GluIleHisSerAsnValAspTyrThrValAspLeuLeuAsnAlaSer610615620AspPheArgGluAsnLysIleHisThrGlyTrpLeuAspThrArgIle625630635640AlaMetArgValGlnAlaGluArgProProTrpTyrIleSerValVal645650655GlyGlyAlaLeuTyrLysThrValThrAlaAsnThrAlaThrValSer660665670AspTyrValGlyTyrLeuThrLysGlyGlnIleProProLysHisIle675680685SerLeuValTyrThrThrValAlaLeuAsnIleAspGlyLysLysTyr690695700ThrIleAspThrValArgSerGlyHisGlySerTyrArgLeuArgMet705710715720AsnGlySerThrValAspAlaAsnValGlnIleLeuCysAspGlyGly725730735LeuLeuMetGlnLeuAspGlyAsnSerHisValIleTyrAlaGluGlu740745750GluAlaSerGlyThrArgLeuLeuIleAspGlyLysThrCysMetLeu755760765GlnAsnAspHisAspProSerLysLeuLeuAlaGluThrProCysLys770775780LeuLeuArgPheLeuValAlaAspGlyAlaHisValAspAlaAspVal785790795800ProTyrAlaGluValGluValMetLysMetCysMetProLeuLeuSer805810815ProAlaSerGlyValIleHisValValMetSerGluGlyGlnAlaMet820825830GlnAlaGlyAspLeuIleAlaArgLeuAspLeuAspAspProSerAla835840845ValLysArgAlaGluProPheGluAspThrPheProGlnMetGlyLeu850855860ProIleAlaAlaSerGlyGlnValHisLysLeuCysAlaAlaSerLeu865870875880AsnAlaCysArgMetIleLeuAlaGlyTyrGluHisAspIleAspLys885890895ValValProGluLeuValTyrCysLeuAspThrProGluLeuProPhe900905910LeuGlnTrpGluGluLeuMetSerValLeuAlaThrArgLeuProArg915920925AsnLeuLysSerGluLeuGluGlyLysTyrGluGluTyrLysValLys930935940PheAspSerGlyIleIleAsnAspPheProAlaAsnMetLeuArgVal945950955960IleIleGluGluAsnLeuAlaCysGlySerGluLysGluLysAlaThr965970975AsnGluArgLeuValGluProLeuMetSerLeuLeuLysSerTyrGlu980985990GlyGlyArgGluSerHisAlaHisPheValValLysSerLeuPheGlu99510001005GluTyrLeuTyrValGluGluLeuPheSerAspGlyIleGlnSerAsp101010151020ValIleGluArgLeuArgLeuGlnHisSerLysAspLeuGlnLysVal1025103010351040ValAspIleValLeuSerHisGlnSerValArgAsnLysThrLysLeu104510501055IleLeuLysLeuMetGluSerLeuValTyrProAsnProAlaAlaTyr106010651070ArgAspGlnLeuIleArgPheSerSerLeuAsnHisLysAlaTyrTyr107510801085LysLeuAlaLeuLysAlaSerGluLeuLeuGluGlnThrLysLeuSer109010951100GluLeuArgAlaArgIleAlaArgSerLeuSerGluLeuGluMetPhe1105111011151120ThrGluGluSerLysGlyLeuSerMetHisLysArgGluIleAlaIle112511301135LysGluSerMetGluAspLeuValThrAlaProLeuProValGluAsp114011451150AlaLeuIleSerLeuPheAspCysSerAspThrThrValGlnGlnArg115511601165ValIleGluThrTyrIleAlaArgLeuTyrGlnProHisLeuValLys117011751180AspSerIleLysMetLysTrpIleGluSerGlyValIleAlaLeuTrp1185119011951200GluPheProGluGlyHisPheAspAlaArgAsnGlyGlyAlaValLeu120512101215GlyAspLysArgTrpGlyAlaMetValIleValLysSerLeuGluSer122012251230LeuSerMetAlaIleArgPheAlaLeuLysGluThrSerHisTyrThr123512401245SerSerGluGlyAsnMetMetHisIleAlaLeuLeuGlyAlaAspAsn125012551260LysMetHisIleIleGlnGluSerGlyAspAspAlaAspArgIleAla1265127012751280LysLeuProLeuIleLeuLysAspAsnValThrAspLeuHisAlaSer128512901295GlyValLysThrIleSerPheIleValGlnArgAspGluAlaArgMet130013051310ThrMetArgArgThrPheLeuTrpSerAspGluLysLeuSerTyrGlu131513201325GluGluProIleLeuArgHisValGluProProLeuSerAlaLeuLeu133013351340GluLeuAspLysLeuLysValLysGlyTyrAsnGluMetLysTyrThr1345135013551360ProSerArgAspArgGlnTrpHisIleTyrThrLeuArgAsnThrGlu136513701375AsnProLysMetLeuHisArgValPhePheArgThrLeuValArgGln138013851390ProSerValSerAsnLysPheSerSerGlyGlnIleGlyAspMetGlu139514001405ValGlySerAlaGluGluProLeuSerPheThrSerThrSerIleLeu141014151420ArgSerLeuMetThrAlaIleGluGluLeuGluLeuHisAlaIleArg1425143014351440ThrGlyHisSerHisMetTyrLeuHisValLeuLysGluGlnLysLeu144514501455LeuAspLeuValProValSerGlyAsnThrValLeuAspValGlyGln146014651470AspGluAlaThrAlaTyrSerLeuLeuLysGluMetAlaMetLysIle147514801485HisGluLeuValGlyAlaArgMetHisHisLeuSerValCysGlnTrp149014951500GluValLysLeuLysLeuAspCysAspGlyProAlaSerGlyThrTrp1505151015151520ArgIleValThrThrAsnValThrSerHisThrCysThrValAspIle152515301535TyrArgGluMetGluAspLysGluSerArgLysLeuValTyrHisPro154015451550AlaThrProAlaAlaGlyProLeuHisGlyValAlaLeuAsnAsnPro155515601565TyrGlnProLeuSerValIleAspLeuLysArgCysSerAlaArgAsn157015751580AsnArgThrThrTyrCysTyrAspPheProLeuAlaPheGluThrAla1585159015951600ValArgLysSerTrpSerSerSerThrSerGlyAlaSerLysGlyVal160516101615GluAsnAlaGlnCysTyrValLysAlaThrGluLeuValPheAlaAsp162016251630LysHisGlySerTrpGlyThrProLeuValGlnMetAspArgProAla163516401645GlyLeuAsnAspIleGlyMetValAlaTrpThrLeuLysMetSerThr165016551660ProGluPheProSerGlyArgGluIleIleValValAlaAsnAspIle1665167016751680ThrPheArgAlaGlySerPheGlyProArgGluAspAlaPhePheGlu168516901695AlaValThrAsnLeuAlaCysGluLysLysLeuProLeuIleTyrLeu170017051710AlaAlaAsnSerGlyAlaArgIleGlyIleAlaAspGluValLysSer171517201725CysPheArgValGlyTrpSerAspAspGlySerProGluArgGlyPhe173017351740GlnTyrIleTyrLeuSerGluGluAspTyrAlaArgIleGlyThrSer1745175017551760ValIleAlaHisLysMetGlnLeuAspSerGlyGluIleArgTrpVal176517701775IleAspSerValValGlyLysGluAspGlyLeuGlyValGluAsnIle178017851790HisGlySerAlaAlaIleAlaSerAlaTyrSerArgAlaTyrLysGlu179518001805ThrPheThrLeuThrPheValThrGlyArgThrValGlyIleGlyAla181018151820TyrLeuAlaArgLeuGlyIleArgCysIleGlnArgLeuAspGlnPro1825183018351840IleIleLeuThrGlyTyrSerAlaLeuAsnLysLeuLeuGlyArgGlu184518501855ValTyrSerSerHisMetGlnLeuGlyGlyProLysIleMetAlaThr186018651870AsnGlyValValHisLeuThrValSerAspAspLeuGluGlyValSer187518801885AsnIleLeuArgTrpLeuSerTyrValProAlaTyrIleGlyGlyPro189018951900LeuProValThrThrProLeuAspProProAspArgProValAlaTyr1905191019151920IleProGluAsnSerCysAspProArgAlaAlaIleArgGlyValAsp192519301935AspSerGlnGlyLysTrpLeuGlyGlyMetPheAspLysAspSerPhe194019451950ValGluThrPheGluGlyTrpAlaLysThrValValThrGlyArgAla195519601965LysLeuGlyGlyIleProValGlyValIleAlaValGluThrGlnThr197019751980MetMetGlnThrIleProAlaAspProGlyGlnLeuAspSerArgGlu1985199019952000GlnSerValProArgAlaGlyGlnValTrpPheProAspSerAlaThr200520102015LysThrAlaGlnAlaLeuLeuAspPheAsnArgGluGlyLeuProLeu202020252030PheIleLeuAlaAsnTrpArgGlyPheSerGlyGlyGlnArgAspLeu203520402045PheGluGlyIleLeuGlnAlaGlySerThrIleValGluAsnLeuArg205020552060ThrTyrAsnGlnProAlaPheValTyrIleProMetAlaAlaGluLeu2065207020752080ArgGlyGlyAlaTrpValValValAspSerLysIleAsnProAspArg208520902095IleGluCysTyrAlaGluArgThrAlaLysGlyAsnValLeuGluPro210021052110GlnGlyLeuIleGluIleLysPheArgSerGluGluLeuGlnAspCys211521202125MetSerArgLeuAspProThrLeuIleAspLeuLysAlaLysLeuGlu213021352140ValAlaAsnLysAsnGlySerAlaAspThrLysSerLeuGlnGluAsn2145215021552160IleGluAlaArgThrLysGlnLeuMetProLeuTyrThrGlnIleAla216521702175IleArgPheAlaGluLeuHisAspThrSerLeuArgMetAlaAlaLys218021852190GlyValIleLysLysValValAspTrpGluGluSerArgSerPhePhe219522002205TyrLysArgLeuArgArgArgIleSerGluAspValLeuAlaLysGlu221022152220IleArgAlaValAlaGlyGluGlnPheSerHisGlnProAlaIleGlu2225223022352240LeuIleLysLysTrpTyrSerAlaSerHisAlaAlaGluTrpAspAsp224522502255AspAspAlaPheValAlaTrpMetAspAsnProGluAsnTyrLysAsp226022652270TyrIleGlnTyrLeuLysAlaGlnArgValSerGlnSerLeuSerSer227522802285LeuSerAspSerSerSerAspLeuGlnAlaLeuProGlnGlyLeuSer229022952300MetLeuLeuAspLysMetAspProSerArgArgAlaGlnLeuValGlu2305231023152320GluIleArgLysValLeuGly23252102321125212DNA213ACCase野生型序列ACC-CF引物ArtificialSequence400232102421125212DNA213ACCase野生型序列ACC-CR引物ArtificialSequence400242102521128212DNA213ACCase-5387突变位点ACC5387-MutF引物ArtificialSequence400252102621128212DNA213ACCase-5387突变位点ACC5387-MutR引物ArtificialSequence400262102721128212DNA213ACCase-5389突变位点ACC5389-MutF引物ArtificialSequence400272102821128212DNA213ACCase-5389突变位点ACC5389-MutR引物ArtificialSequence400282102921128212DNA213ACCase-5504突变位点ACC5504-MutF引物ArtificialSequence400292103021128212DNA213ACCase-5504突变位点ACC5504-MutR引物ArtificialSequence400302103121128212DNA213ACCase-5623突变位点ACC5623-MutF引物ArtificialSequence400312103221128212DNA213ACCase-5623突变位点ACC5623-MutR引物ArtificialSequence400322103321130212DNA213ACCase-6028突变位点ACC6028-MutF引物ArtificialSequence400332103421129212DNA213ACCase-6028突变位点ACC6028-MutF引物ArtificialSequence400342103521130212DNA213ACCase-6030突变位点ACC6030-MutF引物ArtificialSequence400352103621129212DNA213ACCase-6030突变位点ACC6030-MutR引物ArtificialSequence400362103721128212DNA213ACCase-6149突变位点ACC6149-MutF引物ArtificialSequence400372103821128212DNA213ACCase-6149突变位点ACC6149-MutR引物ArtificialSequence400382103921128212DNA213ACCase-6178突变位点ACC6178-MutF引物ArtificialSequence400392104021128212DNA213ACCase-6178突变位点ACC6178-MutR引物ArtificialSequence400402104121128212DNA213ACCase-6209突变位点ACC6209-MutF引物ArtificialSequence400412104221128212DNA213ACCase-6209突变位点ACC6209-MutR引物ArtificialSequence400422104321130212DNA213ACCase-6316突变位点ACC6316-MutF引物ArtificialSequence400432104421128212DNA213ACCase-6316突变位点ACC6316-MutR引物ArtificialSequence40044

权利要求：1.一种水稻ACCase突变型蛋白，所述ACCase突变型蛋白的氨基酸序列存在以下任意一种或多种突变：其对应于野生型水稻ACCase的氨基酸序列的第1796位、第1797位、第1835位、第1875位、第2010位、第2050位、第2060位、第2070位和第2106位发生突变。2.一种水稻ACCase突变型蛋白，包括：（a）其氨基酸序列选自由SEQIDNO.2、SEQIDNO.4、SEQIDNO.6、SEQIDNO.8、SEQIDNO.10、SEQIDNO.12、SEQIDNO.14、SEQIDNO.16、SEQIDNO.18和SEQIDNO.20组成的组中；或（b）在（a）中的氨基酸序列经过取代和或缺失和或添加一个或多个氨基酸，且具有乙酰辅酶A羧化酶活性的由（a）衍生的蛋白质。3.一种核酸或基因，其编码根据权利要求1~2任一项所述的蛋白。4.根据权利要求3所述的核酸或基因，包括：（i）其编码权利要求1~2任一项所述的蛋白；或（ii）在严格条件下与（a）限定的核苷酸序列杂交且编码具有乙酰辅酶A羧化酶活性的蛋白质的核苷酸序列；或（iii）其核苷酸序列选自由SEQIDNO:1、SEQIDNO:3、SEQIDNO:5、SEQIDNO:7、SEQIDNO:9、SEQIDNO:11、SEQIDNO:13、SEQIDNO:15、SEQIDNO:17和SEQIDNO:19组成的组中。5.一种表达盒、重组载体或细胞，其含有根据权利要求3或4所述的核酸或基因。6.根据权利要求1或2所述的水稻ACCase突变型蛋白、根据权利要求3或4所述的核酸或基因，权利要求5所述的表达盒、重组载体或细胞在植物抗除草剂方面的应用。7.根据权利要求6所述的应用，其特征在于，所述除草剂是喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。8.一种获得具有除草剂抗性的植物的方法，其特征在于，包括如下步骤：1）使植物包含根据权利要求3或4所述的核酸或基因；或2）使植物表达根据权利要求1或2所述的水稻ACCase突变型蛋白。9.根据权利要求8所述的方法，其特征在于，其包括crispr基因编辑、转基因、杂交、回交或无性繁殖步骤。10.一种鉴定植物的方法，其中所述植物是包含根据权利要求3或4所述的核酸或基因的植物、表达根据权利要求1~2之任一所述的蛋白的植物或由根据权利要求8~9之任一所述的方法获得的植物，其特征在于，包括以下步骤：1测定所述植物是否包含根据权利要3或4所述的核酸或基因；或，2测定所述植物是否表达根据权利要求1~2之任一所述的蛋白。11.一种控制杂草的方法，其特征在于，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含根据权利要求3或4所述的核酸或基因或根据权利要求5所述的表达盒、重组载体或细胞，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。12.一种用于保护植物免受由除草剂引起的损伤的方法，其特征在于，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含根据权利要求3或4所述的核酸或基因或将权利要求5所述的表达盒、重组载体导入植物，导入后的植物产生除草剂抗性蛋白，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。

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