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申请/专利权人：司法鉴定科学研究院

摘要：本发明公开了一种同时检测STR基因座和SNP位点的试剂盒及其使用方法，该试剂盒包括用于扩增如下132个STR基因座和306个SNP位点的扩增引物，以及用于识别如下132个STR基因座和306个SNP位点的探针。本发明的试剂盒能够同时检测438个遗传标记132个STR基因座和306个SNP位点，极大地提高了个体识别和复杂亲缘关系的鉴别能力。本发明基于探针捕获检测STR和SNP分型的方法，不但分析效率高，而且更具成本效益，结果可靠，可广泛应用于法庭科学中的DNA鉴定。

主权项：1.一种同时检测STR基因座和SNP位点的试剂盒，其特征在于，所述试剂盒包括用于扩增如下132个STR基因座和306个SNP位点的扩增引物，以及用于识别如下132个STR基因座和306个SNP位点的探针；其中，132个STR基因座为：D1S1171、D1S1679、D1S1677、D1S1627、D1S1656、D10S2325、D10S1237、D10S1248、D10S1435、D11S4463、D11S1304、D11S2368、TH01、CD4、D12ATA63、D12S391、VWA、D13S325、D13S317、D13S1492、D14S1434、D14S608、D14S1426、PENTAE、D15S659、D15S822、D16S3253、D16S539、D16S753、D17S1290、D17S975、D17S976、D17S1294、D17S974、D18S1364、D18S1270、D18S51、D18S535、D18S853、D19S253、D19S433、D2S1360、D2S1338、D2S441、D2S1772、D2S1776、D2S427、D2S1242、TPOX、D20S161、D20S470、D20S1082、D20S482、D21S1432、D21S11、D21S1270、PENTAD、D21S1437、D21S2055、D22S689、D22S534、D22GATA198B05、D22S1045、D3S1358、D3S1744、D3S4554、D3S3051、D3S4529、D3S4545、D3S1766、D3S2452、D3S2387、D3S2388、D3S2406、D3S2402、D4S2366、D4S2404、D4S2408、GABRB15、FGA、D5S818、CSF1PO、D5S2500、D5S2503、D5S1457、D6S474、D6S477、D6S1017、D6S1027、D7S820、D7S3048、D7S2201、D7S1517、D8S1115、D8S1132、D8S1179、D8S639、LPL、D9S324、D9S925、D9S1118、D9S304、D9S2157、D9S1122、DXS10163、DXS6789、DXS10161、DXS981、DXS9902、DXS10103、DXS10147、GATA172D05、GATA31E08、HPRTB、DXS6795、DXS6797、DXS6807、DXS6810、DXS7129、DXS7130、DXS9907、DYS533、DYS570、DYS19、DYS390、DYS391、DYS392、DYS425、DYS436、DYS456、DYS458、DYS460；306个SNP位点为：rs730123、rs6681639、rs6022、rs7531163、rs859400、rs647347、rs10921915、rs1049607、rs10779650、rs35396665、rs12042900、rs10925256、rs1413212、rs7517833、rs595961、rs12718433、rs75930208、rs7373、rs1779866、rs2986742、rs7354930、rs681968、rs1281611、rs2991717、rs6671669、rs2799067、rs11191355、rs8946、rs10828545、rs3817419、rs7921673、rs10900297、rs7903315、rs2680327、rs4414169、rs3758626、rs10824095、rs1059046、rs10887346、rs10881855、rs1977640、rs2566913、rs10502151、rs693147、rs2027767、rs2711823、rs7945310、rs10501269、rs3829940、rs4939141、rs7932775、rs4944946、rs11604536、rs2196168、rs794533、rs3751000、rs7929050、rs2764、rs883079、rs1007469、rs478347、rs7962675、rs1909149、rs11171675、rs10747783、rs7486100、rs11178999、rs10506847、rs10459171、rs2107612、rs959897、rs1058083、rs1865350、rs9515124、rs3751385、rs9319336、rs689338、rs2677595、rs9598152、rs7321823、rs495593、rs12856670、rs319522、rs8003942、rs1079375、rs5807790、rs7159343、rs2229869、rs946065、rs33943216、rs3759753、rs165932、rs1004667、rs229829、rs879803、rs4530104、rs2898985、rs700518、rs3848117、rs11558748、rs12442886、rs698500、rs7169902、rs550397、rs4781878、rs567617218、rs17822931、rs4315319、rs1126933、rs8056865、rs7205345、rs1382387、rs8074840、rs1046321、rs17855579、rs189899、rs1055086、rs236514、rs4789193、rs8078417、rs2510344、rs7232841、rs8089593、rs1058396、rs3819122、rs4801083、rs17176581、rs6565924、rs9951171、rs1108414、rs2285513、rs7367、rs2014576、rs2547319、rs7259841、rs2071347、rs1519654、rs12622186、rs9005、rs11888583、rs2716639、rs2920059、rs7597826、rs164605、rs1540482、rs10497191、rs12613075、rs12464448、rs850889、rs13019672、rs13113、rs2242074、rs4074882、rs2068834、rs2372009、rs10203786、rs13419896、rs12617928、rs2561892、rs6742876、rs4777、rs13400937、rs1078004、rs2113418、rs2567608、rs734111、rs3215567、rs2868362、rs1000551、rs994487、rs1014803、rs2073383、rs2040411、rs813449、rs2399332、rs1135750、rs876755、rs6439466、rs1586861、rs1131925、rs150205、rs10470510、rs2270968、rs1017953、rs3747669、rs11128935、rs4493387、rs6802739、rs7374458、rs3774729、rs6123、rs2765、rs1405154、rs10019345、rs11722834、rs167503、rs1396009、rs218804、rs6857303、rs10024103、rs10517844、rs1050145、rs36029731、rs55905558、rs10034991、rs4832887、rs225165、rs270238、rs12640116、rs28675771、rs1043218、rs344140、rs385194、rs10005781、rs412683、rs1898625、rs7703985、rs2162739、rs10079352、rs39897、rs13182883、rs6191、rs1820795、rs11576001、rs332811、rs295999、rs251934、rs446003、rs641437、rs37369、rs2672757、rs10072283、rs2406113、rs2928231、rs2371382、rs277984、rs460267、rs4518370、rs3763070、rs4946582、rs1884255、rs13218440、rs2503107、rs9493627、rs1360947、rs9373491、rs9383976、rs9356399、rs10949483、rs1206523、rs1051952、rs719856、rs11242909、rs6933870、rs6934646、rs2305837、rs818269、rs7767965、rs1030428、rs2747737、rs7779997、rs2285914、rs10487320、rs41736、rs2305324、rs321198、rs2272251、rs740136、rs1233555、rs991471、rs917115、rs36170987、rs1050721、rs4723858、rs2053315、rs6973042、rs1860469、rs10614、rs12386695、rs1403180、rs1515685、rs298612、rs1383474、rs763869、rs10092983、rs2292975、rs10100663、rs3735973、rs4237030、rs2912016、rs7814747、rs28435921、rs7831841、rs2308962、rs13279230、rs1063110、rs2274159、rs7049164、rs2781116、rs1360288、rs2236547、rs7871735、rs10973637、rs4322086、rs6559311、rs8192689。

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